Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocyte

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F09%3A31165" target="_blank" >RIV/60460709:41210/09:31165 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocyte

Popis výsledku v původním jazyce

In fully-grown pig oocytes, meiotic maturation in vitro is retarded by inhibition ofhistone deacetylases by trichostatin A (TSA). In growing oocytes with partial meiotic competence, culture with TSA has no significant effect on the meitic maturation. Growing oocytes treated with TSA mature mainly to the stage of metaphase I. The ratio of oocytes maturing tothe stage of metaphase II is very limited. After transient exposure to TSA, the maturation of growing oocytes with partial meiotic competence takes adifferent course. When these oocytes are first cultured in a TSA-free medium, then cultured for another 24 hours with 100 nM TSA, and finally again inaTSA-free medium for 24 hours, the ratio of oocytes maturing to the stage of metaphase II significantlyincreases reaching 59%. When oocytes were cultured for the same length of time without transient exposure to TSA, only 19% matured to the stage of metaphase II. Those oocytes which matured to the stage of metaphase II after transient exp

Název v anglickém jazyce

Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocyte

Popis výsledku anglicky

In fully-grown pig oocytes, meiotic maturation in vitro is retarded by inhibition ofhistone deacetylases by trichostatin A (TSA). In growing oocytes with partial meiotic competence, culture with TSA has no significant effect on the meitic maturation. Growing oocytes treated with TSA mature mainly to the stage of metaphase I. The ratio of oocytes maturing tothe stage of metaphase II is very limited. After transient exposure to TSA, the maturation of growing oocytes with partial meiotic competence takes adifferent course. When these oocytes are first cultured in a TSA-free medium, then cultured for another 24 hours with 100 nM TSA, and finally again inaTSA-free medium for 24 hours, the ratio of oocytes maturing to the stage of metaphase II significantlyincreases reaching 59%. When oocytes were cultured for the same length of time without transient exposure to TSA, only 19% matured to the stage of metaphase II. Those oocytes which matured to the stage of metaphase II after transient exp

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QG50052" target="_blank" >QG50052: Reprodukční biotechnologie u prasat</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zygote

ISSN

0967-1994

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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