Exprese nestrukturálních proteinů mop-top viru bramboru v bakteriálním systému
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A8778" target="_blank" >RIV/60460709:41210/04:8778 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Expression of non-structural proteins of Potato mop-top virus in procaryotic system
Popis výsledku v původním jazyce
PMTV RNA 1 was divided into 6 parts with the length 1 kb aproximately, and these fragments were inserted into pUC-57A suitable for sequencing. The obtained sequences showed high homology (GenBank AY196959). All fragments were inserted into different expression vectors. First of all we used the non-tagged expression vectors, PMPM-Ω-4, 5, 6; pET-21a+ and pTH. To facilitate detection of expressed proteins we used tagged vectors - either with His tag or glutation S-transferase (GST). GST fusion proteinwas performed in vectors pGEX-4T-1, 2 and pGEX-KG alowing the removing of tag by thrombin. Proteins with His tag were induced in vectors pQE-40 and pQE-60. The highest yield of expressed protein was obtained from pGEX-4T-2 in E. coli BL 21. Disadvantageof this aproach was uncomplete cleavage of GST by thrombin. We consider pQE-40 and pQE-60 as more suitable vectors for the purpose of production of polyclonal antibodies against PMTV proteins, because sequence of tag 6xHis has not been f
Název v anglickém jazyce
Expression of non-structural proteins of Potato mop-top virus in procaryotic system
Popis výsledku anglicky
PMTV RNA 1 was divided into 6 parts with the length 1 kb aproximately, and these fragments were inserted into pUC-57A suitable for sequencing. The obtained sequences showed high homology (GenBank AY196959). All fragments were inserted into different expression vectors. First of all we used the non-tagged expression vectors, PMPM-Ω-4, 5, 6; pET-21a+ and pTH. To facilitate detection of expressed proteins we used tagged vectors - either with His tag or glutation S-transferase (GST). GST fusion proteinwas performed in vectors pGEX-4T-1, 2 and pGEX-KG alowing the removing of tag by thrombin. Proteins with His tag were induced in vectors pQE-40 and pQE-60. The highest yield of expressed protein was obtained from pGEX-4T-2 in E. coli BL 21. Disadvantageof this aproach was uncomplete cleavage of GST by thrombin. We consider pQE-40 and pQE-60 as more suitable vectors for the purpose of production of polyclonal antibodies against PMTV proteins, because sequence of tag 6xHis has not been f
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
GF - Choroby, škůdci, plevely a ochrana rostlin
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2004
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Proceeding of the XVI. Slovak and Czech Plant Protection Conference
ISBN
1335-258X
ISSN
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e-ISSN
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Počet stran výsledku
3
Strana od-do
257-259
Název nakladatele
Slovak Agricultural Universitty in Nitra
Místo vydání
Nitra
Místo konání akce
Nitra
Datum konání akce
16. 9. 2003
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
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