Význam NO-syntázy a oxidu dusnatého pro meiotické zrání oocytu prasete

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11536" target="_blank" >RIV/60460709:41210/05:11536 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nitric Oxide Synthase and Nitric Oxide Importance for Meiotic Maturation of Pig Oocytes

Popis výsledku v původním jazyce

Nitric oxide synthase (NOS) has been considered as a highly regulated enzyme that can produce nitric oxide (NO) from molecular oxygen and L-arginine. Three isoforms of NOS are known neuronal, inducible and endothelial. NO is an important signalling molecule that mediates many physiological processes. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation by NO production. The expression of NOS was studied in three stages of porcine oocytes meiotic maturation, germinal vesicle (0 h), first meiotic metaphase (24 h) and second meiotic metaphase stage (48 h), using western blotting method. The activity of NOS was observed by measurement of NO metabolites, nitrites and nitrates, with Griess reagents. Two NOS isoforms were detected in porcine oocytes: inducible and endothelial nitric oxide synthase. These isoforms were detected in all three stages of porcine oocyte meiotic maturation. The results of NO measurement showed that the amount of NO metabol

Název v anglickém jazyce

Nitric Oxide Synthase and Nitric Oxide Importance for Meiotic Maturation of Pig Oocytes

Popis výsledku anglicky

Nitric oxide synthase (NOS) has been considered as a highly regulated enzyme that can produce nitric oxide (NO) from molecular oxygen and L-arginine. Three isoforms of NOS are known neuronal, inducible and endothelial. NO is an important signalling molecule that mediates many physiological processes. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation by NO production. The expression of NOS was studied in three stages of porcine oocytes meiotic maturation, germinal vesicle (0 h), first meiotic metaphase (24 h) and second meiotic metaphase stage (48 h), using western blotting method. The activity of NOS was observed by measurement of NO metabolites, nitrites and nitrates, with Griess reagents. Two NOS isoforms were detected in porcine oocytes: inducible and endothelial nitric oxide synthase. These isoforms were detected in all three stages of porcine oocyte meiotic maturation. The results of NO measurement showed that the amount of NO metabol

Druh

D - Stať ve sborníku

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/GD523%2F03%2FH076" target="_blank" >GD523/03/H076: Zvýšení metodologické úrovně a teoretického vzdělání studentů akreditovaného DSP 4103V Zootechnika - perspektivního stud. oboru obecná zootechnika</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Journal of Applied Biomedicine

ISBN

1214-021X

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

8-8

Název nakladatele

Biologická fakulta

Místo vydání

České Budějovice

Místo konání akce

České Budějovice

Datum konání akce

26. 10. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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