Úloha NO-syntázy na meiotické zrání prasečích oocytů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7178" target="_blank" >RIV/60460709:41210/04:7178 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Role of Nitric Oxide Synthase in Porcine Oocytes Meiotic Maturation

Popis výsledku v původním jazyce

Nitric oxide Synthase (NOS) has been considered as a highly regulated enzyme that can produce Nitric oxide, which is known as important signalling molecule. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation.In porcine oocytes were detected two NOS isoforms (from three known) NOS II and NOS III at all three studied stages (germinal vesicle, first and second meiotic metaphase). During the culture the amount of NO significantly increased with the stage of meiotic maturation. The addition of NOS inhibitor (L-NAME) significantly decreased the rate of oocytes at the second meiotic metaphase stage. Inactive NOS inhibitor conformer D-NAME did not affect the oocytes maturation. Our results indicate that NOS is involved in process of porcine oocyte meiotic maturation by producing NO.

Název v anglickém jazyce

The Role of Nitric Oxide Synthase in Porcine Oocytes Meiotic Maturation

Popis výsledku anglicky

Nitric oxide Synthase (NOS) has been considered as a highly regulated enzyme that can produce Nitric oxide, which is known as important signalling molecule. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation.In porcine oocytes were detected two NOS isoforms (from three known) NOS II and NOS III at all three studied stages (germinal vesicle, first and second meiotic metaphase). During the culture the amount of NO significantly increased with the stage of meiotic maturation. The addition of NOS inhibitor (L-NAME) significantly decreased the rate of oocytes at the second meiotic metaphase stage. Inactive NOS inhibitor conformer D-NAME did not affect the oocytes maturation. Our results indicate that NOS is involved in process of porcine oocyte meiotic maturation by producing NO.

Druh

D - Stať ve sborníku

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Výzkum v chovu skotu

ISBN

0139-7265

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

44-45

Název nakladatele

Výzkumný ústav pro chov skotu, s. r. o. Rapotín

Místo vydání

Rapotín

Místo konání akce

Velké Losiny

Datum konání akce

16. 9. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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