Nitric oxide and meiotic competence of porcine oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F11%3A51054" target="_blank" >RIV/60460709:41210/11:51054 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027014:_____/11:#0001458

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S1751731111000565" target="_blank" >http://dx.doi.org/10.1017/S1751731111000565</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1751731111000565" target="_blank" >10.1017/S1751731111000565</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nitric oxide and meiotic competence of porcine oocytes

Popis výsledku v původním jazyce

Nitric oxide (NO), produced by NO-Synthase (NOS) enzyme can fulfil a regulatory role in growing period of porcine oocytes. The objective of this work was to ascertain the role of NO in the growth phase of pig oocytes and its influence on the acquisitionof meiotic competence. We demonstrated that the selective competitive iNOS inhibitor, aminoguanidine, and also the nonselective NOS inhibitor L-NAME, block meiotic maturation of oocytes. NOS inhibitors influence even competent oocytes in the first stageof meiotic metaphase. However, blockage is less effective than at the beginning of meiotic maturation. The number of parthenogenetically activated competent oocytes greatly increased in a pure medium after inhibitor reversion. A large quantity of NO externally added to the in vitro cultivation environment disrupts the viability of oocytes. The effectiveness of the inhibitor can be reversed in oocytes by an NO donor in a very low concentration.

Název v anglickém jazyce

Nitric oxide and meiotic competence of porcine oocytes

Popis výsledku anglicky

Nitric oxide (NO), produced by NO-Synthase (NOS) enzyme can fulfil a regulatory role in growing period of porcine oocytes. The objective of this work was to ascertain the role of NO in the growth phase of pig oocytes and its influence on the acquisitionof meiotic competence. We demonstrated that the selective competitive iNOS inhibitor, aminoguanidine, and also the nonselective NOS inhibitor L-NAME, block meiotic maturation of oocytes. NOS inhibitors influence even competent oocytes in the first stageof meiotic metaphase. However, blockage is less effective than at the beginning of meiotic maturation. The number of parthenogenetically activated competent oocytes greatly increased in a pure medium after inhibitor reversion. A large quantity of NO externally added to the in vitro cultivation environment disrupts the viability of oocytes. The effectiveness of the inhibitor can be reversed in oocytes by an NO donor in a very low concentration.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/GD523%2F08%2FH064" target="_blank" >GD523/08/H064: Biotechnologie gamet savců</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal

ISSN

1751-7311

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1398-1405

Kód UT WoS článku

000294412300009

EID výsledku v databázi Scopus

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