Zlepšení vývojové kompetence stárnoucích oocytů po ošetření IGF1 a EGF

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11703" target="_blank" >RIV/60460709:41210/05:11703 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)

Popis výsledku v původním jazyce

There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called ?ageing?, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time.

Název v anglickém jazyce

Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)

Popis výsledku anglicky

There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called ?ageing?, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

300-310

Kód UT WoS článku

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EID výsledku v databázi Scopus

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