Porovnání imunochemických a molekulárních technik pro spolehlivou detekci bakterie, způsobující spálu růžovitých rostlin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11973" target="_blank" >RIV/60460709:41210/05:11973 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of immunochemical and molecular techniques for reliable detection of fire blight bacterium.

Popis výsledku v původním jazyce

Erwinia amylovora [(Burrill) Winslow et al.] (Ea), causing fire blight, is harmful quarantine organism. Fire blight is one of the most serious diseases affecting fruit and ornamental plants of family Rosaceae that has spread in many countries all over the world. Diagnosis of pathogen is based on IFA (indirect fluorescent assay) and bioassay on immature pear fruit assay, but also the other diagnostic techniques are recommended to use for diagnosis of the fire blight organism. PTA-ELISA and IFA, working with polyclonal antibody of Adgen co. (Scotland), were used for testing of 81 isolates of Ea and 38 symptomatic plant samples. Sensitivity of PTA-ELISA, IFA and PCR reached value 106, 105 and 104 CFU/ml, respectively. IFA was not only more sensitive, butalso more specific than PTA-ELISA, because 11 cross-reactions were recorded using IFA in spite of 16 ones in PTA-ELISA. IFA appeared as very sensitive, particularly for screening of plant tissue homogenates. In dilution 1:100 and 1:000, E

Název v anglickém jazyce

Comparison of immunochemical and molecular techniques for reliable detection of fire blight bacterium.

Popis výsledku anglicky

Erwinia amylovora [(Burrill) Winslow et al.] (Ea), causing fire blight, is harmful quarantine organism. Fire blight is one of the most serious diseases affecting fruit and ornamental plants of family Rosaceae that has spread in many countries all over the world. Diagnosis of pathogen is based on IFA (indirect fluorescent assay) and bioassay on immature pear fruit assay, but also the other diagnostic techniques are recommended to use for diagnosis of the fire blight organism. PTA-ELISA and IFA, working with polyclonal antibody of Adgen co. (Scotland), were used for testing of 81 isolates of Ea and 38 symptomatic plant samples. Sensitivity of PTA-ELISA, IFA and PCR reached value 106, 105 and 104 CFU/ml, respectively. IFA was not only more sensitive, butalso more specific than PTA-ELISA, because 11 cross-reactions were recorded using IFA in spite of 16 ones in PTA-ELISA. IFA appeared as very sensitive, particularly for screening of plant tissue homogenates. In dilution 1:100 and 1:000, E

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

From Laboratory to Business. Recent Advances in Plant Biotechnology. České Budějovice

ISBN

80-86778-16-9

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

87-87

Název nakladatele

Institute of Plant Molecular Biology ASCR

Místo vydání

České Budějovice

Místo konání akce

České Budějovice

Datum konání akce

12. 9. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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