Spolehlivost diagnostických technik pro Erwinia amylovora a Clavibacter michiganensis subsp. sepedonicus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F08%3A00000268" target="_blank" >RIV/00027006:_____/08:00000268 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus

Popis výsledku v původním jazyce

Erwinia amylovora (Ea) causing fire blight and Clavibacter michiganensis subsp. sepedoniciis (Cms) causing ring rot of potato belong to the quarantine organisms. Detection and determination of both pathogens using different methods were compared. Commercial MAb were used for Cms and PAb for Ea. All Ea strains were reliably identified by PCR (Bereswill and own primers) after optimisation of PCR protocol and using BIOLOG System. PTA-ELISA and IIF were less reliable because of cross-reacting bacteria associated with Ea in host plants. AH Cms strains were reliably identified by IIF and PCR (Mills and Pastrik primers) regardless of morphological variability. The fluidal Cms strains were more reliably identified using DAS-ELISA and BIOLOG System than less fluidal strains.

Název v anglickém jazyce

Reliability of diagnostic techniques for Erwinia amylovora and Clavibacter michiganensis subsp. sepedonicus

Popis výsledku anglicky

Erwinia amylovora (Ea) causing fire blight and Clavibacter michiganensis subsp. sepedoniciis (Cms) causing ring rot of potato belong to the quarantine organisms. Detection and determination of both pathogens using different methods were compared. Commercial MAb were used for Cms and PAb for Ea. All Ea strains were reliably identified by PCR (Bereswill and own primers) after optimisation of PCR protocol and using BIOLOG System. PTA-ELISA and IIF were less reliable because of cross-reacting bacteria associated with Ea in host plants. AH Cms strains were reliably identified by IIF and PCR (Mills and Pastrik primers) regardless of morphological variability. The fluidal Cms strains were more reliably identified using DAS-ELISA and BIOLOG System than less fluidal strains.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zemdirbyste-Agriculture

ISSN

1392-3196

e-ISSN

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Svazek periodika

95

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

LT - Litevská republika

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000259827600038

EID výsledku v databázi Scopus

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