Omezení v spolehlivosti diagnostických metod pro Erwinia amylovora

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A839" target="_blank" >RIV/00027006:_____/05:839 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Limitation in reliability of fire blight (Erwinia amylovora) diagnostic methods

Popis výsledku v původním jazyce

A total of 20 Erwinia amylovora-like (Ea-like) isolates originating from symptomatic samples of fire blight host plants were analysed.Fourteen of them reacted negatively in immunochemical tests. Six Ea-like isolates reacted positively with a polyclonal antibody for PTA-ELISA (Loewe Co., Germany) and five of them gave positive reaction in indirect If test (Adgen co., Scotland). Three of those isolates were positive in a PCR test with primers AMSbL and AMSbR for E. amylovora. After modification of PCR protocol, putative strains reacted as negatives. Optimization of our PCR test was achieved by finding an optimal annealing temperature for primers. These three strains were identified using the microbial identification system BIOLOG as Pantoea dispersa andP.agglomerans. Identified strains were white variants of species P. dispersa and P. agglomerans that occur less frequently than yellow variants.

Název v anglickém jazyce

Limitation in reliability of fire blight (Erwinia amylovora) diagnostic methods

Popis výsledku anglicky

A total of 20 Erwinia amylovora-like (Ea-like) isolates originating from symptomatic samples of fire blight host plants were analysed.Fourteen of them reacted negatively in immunochemical tests. Six Ea-like isolates reacted positively with a polyclonal antibody for PTA-ELISA (Loewe Co., Germany) and five of them gave positive reaction in indirect If test (Adgen co., Scotland). Three of those isolates were positive in a PCR test with primers AMSbL and AMSbR for E. amylovora. After modification of PCR protocol, putative strains reacted as negatives. Optimization of our PCR test was achieved by finding an optimal annealing temperature for primers. These three strains were identified using the microbial identification system BIOLOG as Pantoea dispersa andP.agglomerans. Identified strains were white variants of species P. dispersa and P. agglomerans that occur less frequently than yellow variants.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Phytopathologia Polonica

ISSN

1230-0462

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

69-72

Kód UT WoS článku

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EID výsledku v databázi Scopus

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