Spolehlivost diagnostických technik pro determinaci Clavibacter michiganensis subsp. sepedonicus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A861" target="_blank" >RIV/00027006:_____/05:861 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus

Popis výsledku v původním jazyce

Clavibacter michiganensis subsp. sepedonicus (further Cms), causing ring rot of potato, is a quarantine bacterium. Diagnosis of Cms is carried out by means of IFAS and bioassay on eggplant (EU directive No. 93/85/EEC), but PCR, ELISA and individual nutrient tests are also recommended. the latter could be substituted using BIOLOG (Biolog inc., USA) or fatty acid analysis (FAA). According to colony morphology, Cms occurs mostly as fluidal (smooth, mucoid), but sometime as intermediate (less fluidal) and nonfluidal (rough, nonmucoid) variants, too. In this study, commercial monoclonal antibodies (Agdia, USA), Pastrik primers PSA-1/PSA-R and dot blot hybridization with digoxigenin-labelled probe were used for determination of Cms strains representing all forms. All Cms strains were reliably identified by IFAS, although apart from typical also atypical cells were sometime recorded in populations of several rough strains. This fluidal Cms strains were more reliably identified using DAS-ELISA

Název v anglickém jazyce

Reliability of diagnostic techniques for determination of Clavibacter michiganensis subsp. sepedonicus

Popis výsledku anglicky

Clavibacter michiganensis subsp. sepedonicus (further Cms), causing ring rot of potato, is a quarantine bacterium. Diagnosis of Cms is carried out by means of IFAS and bioassay on eggplant (EU directive No. 93/85/EEC), but PCR, ELISA and individual nutrient tests are also recommended. the latter could be substituted using BIOLOG (Biolog inc., USA) or fatty acid analysis (FAA). According to colony morphology, Cms occurs mostly as fluidal (smooth, mucoid), but sometime as intermediate (less fluidal) and nonfluidal (rough, nonmucoid) variants, too. In this study, commercial monoclonal antibodies (Agdia, USA), Pastrik primers PSA-1/PSA-R and dot blot hybridization with digoxigenin-labelled probe were used for determination of Cms strains representing all forms. All Cms strains were reliably identified by IFAS, although apart from typical also atypical cells were sometime recorded in populations of several rough strains. This fluidal Cms strains were more reliably identified using DAS-ELISA

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GA - Zemědělská ekonomie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F00%2F0887" target="_blank" >GA522/00/0887: Analýza genetické a patogenní variability v populaci Clavibacter michiganensis subsp. sepedonicus molekulárními technikami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Plant Diseases and Protection

ISSN

1861-3829

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

112

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

16

Strana od-do

1-16

Kód UT WoS článku

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EID výsledku v databázi Scopus

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