Spolehlivost PCR, IFAS a DAS-ELISA pro detekci Clavibacter michiganensis subsp. sepedonicus ve vzorcích hliz brambor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F05%3A%23%23%23%23%23846" target="_blank" >RIV/00027006:_____/05:#####846 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/05:846

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples

Popis výsledku v původním jazyce

Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato, was detected in potato tissue extracts and pure bacterial cultures by means of the polymerase chain reaction (PCR), indirect fluorescent antibody stain(IFAS) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Monoclonal antibodies of company Agdia (USA) were used for IFAS and DAS-ELISA and the primers according to Mills et al. (1997) synthetised by company Biotech (CR) were usedfor PCR. Identities of all Cms strains tested were previously confirmed in the eggplant test as ring rot bacterium. In experiments, 10 % of false positives were recorded in DAS-ELISA, while only 3 % in PCR and IFAS. In assays of pure cultures, Cms wasdetermined at a concentration corresponding to 104 - 103 CFU/ml per PCR and IFAS reactions, and to 105 - 104 CFU/ml per DAS-ELISA. In 18-month-old potato extracts maintained in icebox of refrigerator (-20 oC), the presence of Cms was reliab

Název v anglickém jazyce

Reliability of PCR, IFAS, and DAS-ELISA for detection of Clavibacter michiganensis subsp. sepedonicus in tuber potato samples

Popis výsledku anglicky

Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato, was detected in potato tissue extracts and pure bacterial cultures by means of the polymerase chain reaction (PCR), indirect fluorescent antibody stain(IFAS) and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Monoclonal antibodies of company Agdia (USA) were used for IFAS and DAS-ELISA and the primers according to Mills et al. (1997) synthetised by company Biotech (CR) were usedfor PCR. Identities of all Cms strains tested were previously confirmed in the eggplant test as ring rot bacterium. In experiments, 10 % of false positives were recorded in DAS-ELISA, while only 3 % in PCR and IFAS. In assays of pure cultures, Cms wasdetermined at a concentration corresponding to 104 - 103 CFU/ml per PCR and IFAS reactions, and to 105 - 104 CFU/ml per DAS-ELISA. In 18-month-old potato extracts maintained in icebox of refrigerator (-20 oC), the presence of Cms was reliab

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GA - Zemědělská ekonomie

OECD FORD obor

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Projekt

<a href="/cs/project/QC0048" target="_blank" >QC0048: Molekulární a imunoenzymatická diagnostika vybraných karanténních, regulovaných a hospodářsky škodlivých patogenů rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta fytotechnica et zootechnica

ISSN

1335-258X

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

4

Strana od-do

5-8

Kód UT WoS článku

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EID výsledku v databázi Scopus

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