Možnost detekce nefluidních kmenů Clavibacter michiganensis subsp. sepedonicus v extraktech z bramborových hlíz

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23911" target="_blank" >RIV/00027006:_____/04:#####911 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Possibility to detect non-fluid strains of Clavibacter michiganensis subsp. sepedonicus in potato tuber macerate

Popis výsledku v původním jazyce

Bacterial ring rot disease of potato, caused by Clavibacter michiganensis subsp. sepedonicus (Cms) is a major concern of producers of seed potatoes in the all countries of the world. Current methods of detection of this pathogen rely primarily upon the use of serological procedures including an indirect fluorescent antibody stanining assay (IFAS) and enzyme-linked immunosorbent assay (ELISA). Accurate identification of Cms is especially difficult when population levels are below 10,000 CFU per ml, of plant tissue or millilitre of plant sap. A further complication arises when potato samples contain other bacteria that exhibit cross-reactivity by IFAS and ELISA. Moreover, certain non-fluid variant of Cms that may occur in various natural populations exhibit antigenic variation and escape serological detection. In our tests, potato tubers from samples free of Cms were used. Internal tissue was removed from tubers and homogenized. Cms strains were added to the homogenates to obtain final c

Název v anglickém jazyce

Possibility to detect non-fluid strains of Clavibacter michiganensis subsp. sepedonicus in potato tuber macerate

Popis výsledku anglicky

Bacterial ring rot disease of potato, caused by Clavibacter michiganensis subsp. sepedonicus (Cms) is a major concern of producers of seed potatoes in the all countries of the world. Current methods of detection of this pathogen rely primarily upon the use of serological procedures including an indirect fluorescent antibody stanining assay (IFAS) and enzyme-linked immunosorbent assay (ELISA). Accurate identification of Cms is especially difficult when population levels are below 10,000 CFU per ml, of plant tissue or millilitre of plant sap. A further complication arises when potato samples contain other bacteria that exhibit cross-reactivity by IFAS and ELISA. Moreover, certain non-fluid variant of Cms that may occur in various natural populations exhibit antigenic variation and escape serological detection. In our tests, potato tubers from samples free of Cms were used. Internal tissue was removed from tubers and homogenized. Cms strains were added to the homogenates to obtain final c

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

7th Conference of the European Foundation for Plant Pathology & British Society for Plant Pathology Presidential Meeting 2004, University of Aberdeen, UK, 5th x 10th September 2004. Discovery, Development and Delivery in Plant Pathology.

ISBN

není přiřazeno

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

50-50

Název nakladatele

University os Aberdeen

Místo vydání

University of Aberdeen, United Kingdom

Místo konání akce

University of Aberdeen, United Kingdom

Datum konání akce

5. 9. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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