Využití živných semi-selektivních médií pro izolaci Clavibacter michiganensis subsp. sepedonicus a detekci ELISA metodou

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F04%3A875" target="_blank" >RIV/00027006:_____/04:875 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The use of nutrient semi-selective media for isolation of Clavibacter michiganensis subsp. sepedonicus and detection in ELISA method

Popis výsledku v původním jazyce

Clavibacter michiganensis subsp. sepedonicus (Spieckman & Kotthoff) Davis et al. causes the serious ring rot disease of potato in many potato-growing areas around the world. Immunochemical diagnosis of the ring rot bacterium is based on the use of high-quality monoclonal antibodies among several serological methods, of which ELISA belongs to the most important. Although monoclonal antibodies are able to detect very low concentrations of the target bacterium, Cms usually is not found at concentration of103 cells/ml or less. Therefore new techniques and ways are reached to enhance diagnosis of Cms. Commercial monoclonal antibodies (Agdia, USA) were used for determination and detection of Cms in potato tuber extracts by means of DAS-ELISA (double antibody sandwich enzyme-linked immunosorbent assay) and DASI-ELISA (double antibody sandwich indirect enzyme-linked immunosorbent assay), both with an enrichment step. Four semi-selective media, MTNA, NCP-88, PW 7 and PW 10 were used for cultiv

Název v anglickém jazyce

The use of nutrient semi-selective media for isolation of Clavibacter michiganensis subsp. sepedonicus and detection in ELISA method

Popis výsledku anglicky

Clavibacter michiganensis subsp. sepedonicus (Spieckman & Kotthoff) Davis et al. causes the serious ring rot disease of potato in many potato-growing areas around the world. Immunochemical diagnosis of the ring rot bacterium is based on the use of high-quality monoclonal antibodies among several serological methods, of which ELISA belongs to the most important. Although monoclonal antibodies are able to detect very low concentrations of the target bacterium, Cms usually is not found at concentration of103 cells/ml or less. Therefore new techniques and ways are reached to enhance diagnosis of Cms. Commercial monoclonal antibodies (Agdia, USA) were used for determination and detection of Cms in potato tuber extracts by means of DAS-ELISA (double antibody sandwich enzyme-linked immunosorbent assay) and DASI-ELISA (double antibody sandwich indirect enzyme-linked immunosorbent assay), both with an enrichment step. Four semi-selective media, MTNA, NCP-88, PW 7 and PW 10 were used for cultiv

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F00%2F0887" target="_blank" >GA522/00/0887: Analýza genetické a patogenní variability v populaci Clavibacter michiganensis subsp. sepedonicus molekulárními technikami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta fytotechnica et zootechnica

ISSN

1335-258X

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

3

Strana od-do

141-143

Kód UT WoS článku

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EID výsledku v databázi Scopus

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