Hledání a charkterizace SCAR markerů za účelem molekulární detekce osních nematod Ditylenchus dipsaci

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F06%3A18210" target="_blank" >RIV/60460709:41210/06:18210 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification and evaluation of SCAR markers for molecular detection of the stem and bulb nematode Ditylenchus dipsaci.

Popis výsledku v původním jazyce

Ditylenchus dipsaci, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of control of D. dipsaci is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid and reliable as well as cost effective technique is needed to identification of D. dipsaci in biological samples. This study describes the development of species-specific pairs ofPCR oligonucleotides for detection and identification of the stem nematode D. dipsaci in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all D. dipsaci isolates. The resulting two developed SCAR primer pairs were further tested using as template DNA extracted from collection of twelve healthy plant hosts, but no amplification was observed. The PCR protocol was shown to be quite se

Název v anglickém jazyce

Identification and evaluation of SCAR markers for molecular detection of the stem and bulb nematode Ditylenchus dipsaci.

Popis výsledku anglicky

Ditylenchus dipsaci, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of control of D. dipsaci is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid and reliable as well as cost effective technique is needed to identification of D. dipsaci in biological samples. This study describes the development of species-specific pairs ofPCR oligonucleotides for detection and identification of the stem nematode D. dipsaci in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all D. dipsaci isolates. The resulting two developed SCAR primer pairs were further tested using as template DNA extracted from collection of twelve healthy plant hosts, but no amplification was observed. The PCR protocol was shown to be quite se

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of XVII. Czech and Slovak Plant Protection Conference. September 12-14, 2006,

ISBN

80-213-1564-4

ISSN

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e-ISSN

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Počet stran výsledku

10

Strana od-do

488-497

Název nakladatele

ČZU Praha

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

12. 9. 2006

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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