Iniciace a histologie somatické embryogeneze a organogeneze u Phlox paniculata L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F07%3A23442" target="_blank" >RIV/60460709:41210/07:23442 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L.

Popis výsledku v původním jazyce

Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars ´Fuji´ and ´Starfire´ belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5?1.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplementedwith 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 °C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium w

Název v anglickém jazyce

Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L.

Popis výsledku anglicky

Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars ´Fuji´ and ´Starfire´ belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5?1.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplementedwith 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 °C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium w

Druh

D - Stať ve sborníku

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Propagation of Ornamental Plants. Proc. of the 5th Intern. Conference ?Propagation of Ornamental Plants

ISBN

978-954-90425-4-2

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

70-70

Název nakladatele

SEJANI Publisher

Místo vydání

Sofia, Bulgaria

Místo konání akce

Sofia, Bulgaria

Datum konání akce

5. 9. 2007

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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