Optimální podmínky uchovávání produktu transientně exprimovaných epitopů lidského papilloma viru pomocí virového vektoru odvozeného od X viru bramboru

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A26073" target="_blank" >RIV/60460709:41210/08:26073 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimum storage conditions for produkt of transiently expressed epitopes of Human papillomavirus using Potato virus X-based vector

Popis výsledku v původním jazyce

We describe the optimized conditions of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV-16). Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminal. The construct was cloned into Potato X potexvirus (PVX) based vector and transiently expressed in plants using Agrobacterium tumefaciens mediated inoculation. The effect of storage conditions on the serological activity of L2ACPE7 was studied by ELISA using IgG anti PVX, PVA and L2. Purified L2ACPE7 stored freeze-dried (at -20oC), frozen at various temperatures (-20oC,-70 oC) and at +4oC wer tested. Purified L2ACPE7 was most stable as lyophilized material stored at -20oC. Our study demonstrates suitable way for the storage of plant materiál containing foreign viral epitopes for the purposes of edible vaccination.

Název v anglickém jazyce

Optimum storage conditions for produkt of transiently expressed epitopes of Human papillomavirus using Potato virus X-based vector

Popis výsledku anglicky

We describe the optimized conditions of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV-16). Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminal. The construct was cloned into Potato X potexvirus (PVX) based vector and transiently expressed in plants using Agrobacterium tumefaciens mediated inoculation. The effect of storage conditions on the serological activity of L2ACPE7 was studied by ELISA using IgG anti PVX, PVA and L2. Purified L2ACPE7 stored freeze-dried (at -20oC), frozen at various temperatures (-20oC,-70 oC) and at +4oC wer tested. Purified L2ACPE7 was most stable as lyophilized material stored at -20oC. Our study demonstrates suitable way for the storage of plant materiál containing foreign viral epitopes for the purposes of edible vaccination.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

52

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

3

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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