Transient expression of fusion gene coding for the HPV-16 epitopes fused to the sequence of potyvirus coat protein using different means of inoculation of Nicotiana benthamiana and Brassica rapa, cv. Rapa plants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A26756" target="_blank" >RIV/60460709:41210/08:26756 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/08:00320736

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Transient expression of fusion gene coding for the HPV-16 epitopes fused to the sequence of potyvirus coat protein using different means of inoculation of Nicotiana benthamiana and Brassica rapa, cv. Rapa plants.

Popis výsledku v původním jazyce

We describe the effect of the different means of inoculation of Nicotina benthamiana plants on the amount of transiently expressed fussion gene coding for the Human papillomavirus type 16 (HPV-16) epitopes fused to the sequence of recombinant Potato virus A coat protein (ACP). Epitope derived from minor capsid protein L2 was expressed as an N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The 885 bp construct (named L2ACPE7) was cloned into Potato virus X (PVX) based vector pGR106. In our experiments we compared two different means of agrobacterium-mediated infection in N. benthamiana plants; agroinfection using either hypodermic syringe or vacuum infiltration. Furthemore, we describe the possibilityof L2ACPE7 expression in an edible plant B. rapa, cv. Rapa. The presence and expression of resulting L2ACPE7 in B.rapa infected plants were confirmed by Immuno-Capture Reverse Trascription PCR (ICRT PCR), Plate-Trapped Antigen ELISA (PTA-

Název v anglickém jazyce

Transient expression of fusion gene coding for the HPV-16 epitopes fused to the sequence of potyvirus coat protein using different means of inoculation of Nicotiana benthamiana and Brassica rapa, cv. Rapa plants.

Popis výsledku anglicky

We describe the effect of the different means of inoculation of Nicotina benthamiana plants on the amount of transiently expressed fussion gene coding for the Human papillomavirus type 16 (HPV-16) epitopes fused to the sequence of recombinant Potato virus A coat protein (ACP). Epitope derived from minor capsid protein L2 was expressed as an N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The 885 bp construct (named L2ACPE7) was cloned into Potato virus X (PVX) based vector pGR106. In our experiments we compared two different means of agrobacterium-mediated infection in N. benthamiana plants; agroinfection using either hypodermic syringe or vacuum infiltration. Furthemore, we describe the possibilityof L2ACPE7 expression in an edible plant B. rapa, cv. Rapa. The presence and expression of resulting L2ACPE7 in B.rapa infected plants were confirmed by Immuno-Capture Reverse Trascription PCR (ICRT PCR), Plate-Trapped Antigen ELISA (PTA-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/GA521%2F06%2F0973" target="_blank" >GA521/06/0973: Exprese antigenních papillomových virových epitopů v rostlinách pomocí rostlinného virového vektoru</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

94

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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