Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte aging in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F13%3A61524" target="_blank" >RIV/60460709:41210/13:61524 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte aging in vitro

Popis výsledku v původním jazyce

Oocyte aging is the complex of processes that occur when matured in vitro oocytes are exposed, after reaching the metaphase II stage further in the vitro culture. Aged oocytes remaining at the metaphase II stage, underwent spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) or lysis. The key factor in apoptotic pathway regulation is c-Jun-terminal kinase (JNK), stress kinase from the MAPK kinase family. To investigate the effect of JNK inhibition on porcine oocytes aging, cleavage rate and embryonic development after parthenogenetic activation, DNA fragmentation, and pro-apoptotic factor Bax expression, we cultured in vitro matured oocytes for another 1 to 4 days in the presence of a JNK inhibitor. The inhibition of JNK significantly protected oocytes from the fragmentation (0.0 % of fragmented oocytes under JNK inhibition vs. 13.4% of fragmented oocytes in the control group, second day of aging) and increased the percentage of parthenogenetical

Název v anglickém jazyce

Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte aging in vitro

Popis výsledku anglicky

Oocyte aging is the complex of processes that occur when matured in vitro oocytes are exposed, after reaching the metaphase II stage further in the vitro culture. Aged oocytes remaining at the metaphase II stage, underwent spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) or lysis. The key factor in apoptotic pathway regulation is c-Jun-terminal kinase (JNK), stress kinase from the MAPK kinase family. To investigate the effect of JNK inhibition on porcine oocytes aging, cleavage rate and embryonic development after parthenogenetic activation, DNA fragmentation, and pro-apoptotic factor Bax expression, we cultured in vitro matured oocytes for another 1 to 4 days in the presence of a JNK inhibitor. The inhibition of JNK significantly protected oocytes from the fragmentation (0.0 % of fragmented oocytes under JNK inhibition vs. 13.4% of fragmented oocytes in the control group, second day of aging) and increased the percentage of parthenogenetical

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

535-545

Kód UT WoS článku

000328563000001

EID výsledku v databázi Scopus

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