n vitro stárnutí prasečích oocytů efekt inhibitoru histon deacetylázy trichostatinu A

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A26239" target="_blank" >RIV/60460709:41210/08:26239 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro aging of pig oocytes: effects of the histone deacetylase inhibitor trichostatin A

Popis výsledku v původním jazyce

After in vitro maturation, the unfertilized pig oocytes underwent the process called aging. This process involves typical events such as fragmentation, spontaneous parthenogenetic activation or lysis. Inhibition of histone deacetylase, using its specificinhibitor trichostatin A (TSA), significantly delayed the maturation of pig oocytes cultured in vitro. The aging of oocytes matured under the effect of TSA is the same as the aging in oocytes matured without TSA. The inhibition of histone deacetylase during oocyte aging significantly reduced the percentage of fragmented oocytes (from 30% in untreated oocytes to 9% in oocytes aged under the effect of 100 nM of TSA). Oocytes matured in vitro and subsequently aged for 1 day under the effects of TSA retained their developmental capacity. After parthenogenetic activation, a significantly higher portion (27% vs. 15%) of oocytes developed to the blastocyst stage after 24hour aging under100 nM TSA when compared to oocytes activated after 24hou

Název v anglickém jazyce

In vitro aging of pig oocytes: effects of the histone deacetylase inhibitor trichostatin A

Popis výsledku anglicky

After in vitro maturation, the unfertilized pig oocytes underwent the process called aging. This process involves typical events such as fragmentation, spontaneous parthenogenetic activation or lysis. Inhibition of histone deacetylase, using its specificinhibitor trichostatin A (TSA), significantly delayed the maturation of pig oocytes cultured in vitro. The aging of oocytes matured under the effect of TSA is the same as the aging in oocytes matured without TSA. The inhibition of histone deacetylase during oocyte aging significantly reduced the percentage of fragmented oocytes (from 30% in untreated oocytes to 9% in oocytes aged under the effect of 100 nM of TSA). Oocytes matured in vitro and subsequently aged for 1 day under the effects of TSA retained their developmental capacity. After parthenogenetic activation, a significantly higher portion (27% vs. 15%) of oocytes developed to the blastocyst stage after 24hour aging under100 nM TSA when compared to oocytes activated after 24hou

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zygote

ISSN

0967-1994

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—