CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A76549" target="_blank" >RIV/60460709:41210/18:76549 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/18:00492845 RIV/00216208:11310/18:10382041

Výsledek na webu

<a href="http://dx.doi.org/10.3390/ijms19041236" target="_blank" >http://dx.doi.org/10.3390/ijms19041236</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms19041236" target="_blank" >10.3390/ijms19041236</a>

Jazyk výsledku

angličtina

Název v původním jazyce

CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization

Popis výsledku v původním jazyce

Proteins CD9 and CD81 are members of the tetraspanin superfamily and were detected in mammalian sperm, where they are suspected to form an active tetraspanin web and to participate in sperm egg membrane fusion. The importance of these two proteins during the early stages of fertilization is supported by the complete sterility of CD9 CD81 double null female mice. In this study, the putative mechanism of CD9 CD81 involvement in tetraspanin web formation in sperm and its activity prior to fertilization was addressed. Confocal microscopy and colocalization assay was used to determine a mutual CD9 CD81 localization visualised in detail by super resolution microscopy, and their interaction was address by co-immunoprecipitation. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. During the acrosom

Název v anglickém jazyce

CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization

Popis výsledku anglicky

Proteins CD9 and CD81 are members of the tetraspanin superfamily and were detected in mammalian sperm, where they are suspected to form an active tetraspanin web and to participate in sperm egg membrane fusion. The importance of these two proteins during the early stages of fertilization is supported by the complete sterility of CD9 CD81 double null female mice. In this study, the putative mechanism of CD9 CD81 involvement in tetraspanin web formation in sperm and its activity prior to fertilization was addressed. Confocal microscopy and colocalization assay was used to determine a mutual CD9 CD81 localization visualised in detail by super resolution microscopy, and their interaction was address by co-immunoprecipitation. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. During the acrosom

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

17

Strana od-do

1-17

Kód UT WoS článku

000434978700315

EID výsledku v databázi Scopus

2-s2.0-85045909159