Effects of Various Incubation Conditions on Functional Parameters of Stallion Spermatozoa
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A76958" target="_blank" >RIV/60460709:41210/18:76958 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.2478/sab-2018-0026" target="_blank" >http://dx.doi.org/10.2478/sab-2018-0026</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/sab-2018-0026" target="_blank" >10.2478/sab-2018-0026</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effects of Various Incubation Conditions on Functional Parameters of Stallion Spermatozoa
Popis výsledku v původním jazyce
The objective of our study was to determine the effect of 5 percent of CO2 atmosphere and time of sample dilution on results of in vitro analysis of stallion semen. Frozen-thawed semen from 14 stallions was incubated either in incubator or in a water bath, diluted prior to analysis or immediatelly after thawing. The following qualitative parameters were assessed after thawing T0 and after 30 min T3 motility in 3 sperm subpopulations slow, medium, fast defined by cluster analysis of parameters obtained by Computer Assisted Sperm Analysis, viability, acrosome and mitochondrial integrity. The slow subpopulation was only significantly reduced in diluted samples in CO2 atmosphere at T0 P lower than 0,05. In diluted samples the incubation time sig-nificantly affected distribution of fast, slow, and medium subpopulations in CO2 and CO2 free atmosphere P lower than 0,05, respec-tively. Viability, acrosome and mitochondrial integrity were not affected by CO
Název v anglickém jazyce
Effects of Various Incubation Conditions on Functional Parameters of Stallion Spermatozoa
Popis výsledku anglicky
The objective of our study was to determine the effect of 5 percent of CO2 atmosphere and time of sample dilution on results of in vitro analysis of stallion semen. Frozen-thawed semen from 14 stallions was incubated either in incubator or in a water bath, diluted prior to analysis or immediatelly after thawing. The following qualitative parameters were assessed after thawing T0 and after 30 min T3 motility in 3 sperm subpopulations slow, medium, fast defined by cluster analysis of parameters obtained by Computer Assisted Sperm Analysis, viability, acrosome and mitochondrial integrity. The slow subpopulation was only significantly reduced in diluted samples in CO2 atmosphere at T0 P lower than 0,05. In diluted samples the incubation time sig-nificantly affected distribution of fast, slow, and medium subpopulations in CO2 and CO2 free atmosphere P lower than 0,05, respec-tively. Viability, acrosome and mitochondrial integrity were not affected by CO
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
—
OECD FORD obor
10604 - Reproductive biology (medical aspects to be 3)
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Scientia Agriculturae Bohemica
ISSN
1211-3174
e-ISSN
1805-9430
Svazek periodika
49
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
8
Strana od-do
201-208
Kód UT WoS článku
—
EID výsledku v databázi Scopus
2-s2.0-85054361235