The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41110%2F20%3A83945" target="_blank" >RIV/60460709:41110/20:83945 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60460709:41210/20:83945
Výsledek na webu
<a href="https://doi.org/10.17221/127/2020-CJAS" target="_blank" >https://doi.org/10.17221/127/2020-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/127/2020-CJAS" target="_blank" >10.17221/127/2020-CJAS</a>
Alternativní jazyky
Jazyk výsledku
čeština
Název v původním jazyce
The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers
Popis výsledku v původním jazyce
The aim of this study was to evaluate differences in the presence of sperm subpopulations in frozen-thawed semen in stallions with different freezability. The motility of individual spermatozoa of 24 stallions from 15 breeds was evaluated using computer-assisted sperm analysis (CASA) immediately after thawing (T0) and after 30 min of incubation (T30). In accordance with our previous studies, samples were initially divided based on their total motility into categories of good (GF) and poor (PF) freezers. K-means cluster analysis of kinematic parameters of spermatozoa was used to divide motile sperm into three subpopulations. Analysis of variance was used to evaluate differences in the subpopulations between GF and PF stallions at the times of incubation T0 and T30. Statistically significant differences were found in most kinematic parameters between PF and GF stallions as well as between the times of incubation T0 and T30. Spermatozoa of good freezers are represented more frequently in the fast and me
Název v anglickém jazyce
The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers
Popis výsledku anglicky
The aim of this study was to evaluate differences in the presence of sperm subpopulations in frozen-thawed semen in stallions with different freezability. The motility of individual spermatozoa of 24 stallions from 15 breeds was evaluated using computer-assisted sperm analysis (CASA) immediately after thawing (T0) and after 30 min of incubation (T30). In accordance with our previous studies, samples were initially divided based on their total motility into categories of good (GF) and poor (PF) freezers. K-means cluster analysis of kinematic parameters of spermatozoa was used to divide motile sperm into three subpopulations. Analysis of variance was used to evaluate differences in the subpopulations between GF and PF stallions at the times of incubation T0 and T30. Statistically significant differences were found in most kinematic parameters between PF and GF stallions as well as between the times of incubation T0 and T30. Spermatozoa of good freezers are represented more frequently in the fast and me
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
1805-9309
Svazek periodika
65
Číslo periodika v rámci svazku
11
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
8
Strana od-do
403-410
Kód UT WoS článku
000595926000001
EID výsledku v databázi Scopus
2-s2.0-85097493800