How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41110%2F19%3A79282" target="_blank" >RIV/60460709:41110/19:79282 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60460709:41210/19:79282
Výsledek na webu
<a href="https://www.mdpi.com/2076-2615/9/7/414/htm" target="_blank" >https://www.mdpi.com/2076-2615/9/7/414/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ani9070414" target="_blank" >10.3390/ani9070414</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing
Popis výsledku v původním jazyce
The aim of this study was to evaluate the effect of the addition of two types of seminal plasma (SP) after thawing on the functional characteristics of frozen thawed spermatozoa of poor freezing stallions during prolonged incubation periods. Seminal plasma from stallions with 35-40 percent (standard seminal plasma) and 60-70 percent (above standard seminal plasma, (A-SP)) progressively motile spermatozoa after thawing was used. The motility, kinematic parameters (Computer Assisted Sperm Analysis), distribution of spermatozoa into subpopulations, integrity (carboxyfluorescein diacetate/propidium iodide staining), and functionality (hypo-osmotic swelling (HOS) test) of the spermatozoa plasma membrane were evaluated after thawing (T0) and after 30 min (T30) of incubation at 37 degrees of Celsius. There was no effect of SP addition on spermatozoa motility, but there was a significant positive effect on the kinematic parameters at T0 and T30. The addition of SP significantly increased the percentage of sp
Název v anglickém jazyce
How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing
Popis výsledku anglicky
The aim of this study was to evaluate the effect of the addition of two types of seminal plasma (SP) after thawing on the functional characteristics of frozen thawed spermatozoa of poor freezing stallions during prolonged incubation periods. Seminal plasma from stallions with 35-40 percent (standard seminal plasma) and 60-70 percent (above standard seminal plasma, (A-SP)) progressively motile spermatozoa after thawing was used. The motility, kinematic parameters (Computer Assisted Sperm Analysis), distribution of spermatozoa into subpopulations, integrity (carboxyfluorescein diacetate/propidium iodide staining), and functionality (hypo-osmotic swelling (HOS) test) of the spermatozoa plasma membrane were evaluated after thawing (T0) and after 30 min (T30) of incubation at 37 degrees of Celsius. There was no effect of SP addition on spermatozoa motility, but there was a significant positive effect on the kinematic parameters at T0 and T30. The addition of SP significantly increased the percentage of sp
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Animals
ISSN
2076-2615
e-ISSN
2076-2615
Svazek periodika
9
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
10
Strana od-do
1-10
Kód UT WoS článku
000478611800020
EID výsledku v databázi Scopus
2-s2.0-85069690246