Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A77131" target="_blank" >RIV/60460709:41210/18:77131 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17221/117/2018-CJAS" target="_blank" >http://dx.doi.org/10.17221/117/2018-CJAS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/117/2018-CJAS" target="_blank" >10.17221/117/2018-CJAS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes

Popis výsledku v původním jazyce

The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls, 72 samples) and Czech Fleckvieh (4 bulls, 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0,25 and 0,5 ml), thawing using the slow protocol (P1) was the most appropriate for both obser

Název v anglickém jazyce

Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes

Popis výsledku anglicky

The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls, 72 samples) and Czech Fleckvieh (4 bulls, 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0,25 and 0,5 ml), thawing using the slow protocol (P1) was the most appropriate for both obser

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1210109" target="_blank" >QJ1210109: Inovace zpracování ejakulátu býků a technologických postupů výroby inseminačních dávek vedoucí ke zvýšení jejich oplozovací schopnosti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

—

Svazek periodika

63

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

399-407

Kód UT WoS článku

000445766800003

EID výsledku v databázi Scopus

2-s2.0-85054718772