Stress response of Escherichia coli to essential oil components – insights on low-molecular-weight proteins from MALDI-TOF

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A77999" target="_blank" >RIV/60460709:41210/18:77999 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/18:43915652

Výsledek na webu

<a href="http://dx.doi.org/10.1038/s41598-018-31255-2" target="_blank" >http://dx.doi.org/10.1038/s41598-018-31255-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-018-31255-2" target="_blank" >10.1038/s41598-018-31255-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stress response of Escherichia coli to essential oil components – insights on low-molecular-weight proteins from MALDI-TOF

Popis výsledku v původním jazyce

The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on low-molecular-weight proteins. From 297 m/z features, we identified 94 proteins with important differences in expression among untreated samples, samples treated with EOCs, and samples treated with antibiotics, peroxide, or chlorine. The targets of these treatments obviously differ, even among EOCs. In addition to ribosomal proteins, stress-, membrane- and biofilm-related proteins were affected. These findings may provide a basis for identifying new targets of essential oils and synergies with other antibiotics.

Název v anglickém jazyce

Stress response of Escherichia coli to essential oil components – insights on low-molecular-weight proteins from MALDI-TOF

Popis výsledku anglicky

The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on low-molecular-weight proteins. From 297 m/z features, we identified 94 proteins with important differences in expression among untreated samples, samples treated with EOCs, and samples treated with antibiotics, peroxide, or chlorine. The targets of these treatments obviously differ, even among EOCs. In addition to ribosomal proteins, stress-, membrane- and biofilm-related proteins were affected. These findings may provide a basis for identifying new targets of essential oils and synergies with other antibiotics.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

8

Číslo periodika v rámci svazku

13042

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

000443003800049

EID výsledku v databázi Scopus

2-s2.0-85052671457