Quantification of Firmicutes, Actinobacteria, and Gammaproteobacteria from Bohemian Honey

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A78019" target="_blank" >RIV/60460709:41210/18:78019 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/18:00498909 RIV/00027014:_____/18:N0000295

Výsledek na webu

<a href="http://dx.doi.org/10.2478/sab-2018-0025" target="_blank" >http://dx.doi.org/10.2478/sab-2018-0025</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/sab-2018-0025" target="_blank" >10.2478/sab-2018-0025</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of Firmicutes, Actinobacteria, and Gammaproteobacteria from Bohemian Honey

Popis výsledku v původním jazyce

Honey, which has been used as an ancient remedy for infected wounds, has been shown in laboratory studies to have antimicrobial action against a spectrum of bacteria and fungi. Because very little quantitative information exists on the microbiota of honey, the aim of this study was to quantify the Actinobacteria, Firmicutes, and Gammaproteobacteria groups in samples of honeydew honey and blossom honey from six regions in the Czech Republic, using quantitative realtime PCR analysis with specific primers based on the 16S rRNA gene. Gammaproteobacteria and Firmicutes were clearly the most abundant, predominating Actinobacteria in both types of honey. Most of the Firmicutes were detected in samples from South Bohemia (mean gene copies per 1 g honey 5,6 to 105) and Ústí nad Labem Region (3,7 to 105), which contained the lowest number of Gammaproteobacteria (15,5 to 103). The Actinobacteria were prevalent in samples from Plzeň (4,3 to 103) and Central Bohemia (5,4 to 103), where conversely the Firmicutes w

Název v anglickém jazyce

Quantification of Firmicutes, Actinobacteria, and Gammaproteobacteria from Bohemian Honey

Popis výsledku anglicky

Honey, which has been used as an ancient remedy for infected wounds, has been shown in laboratory studies to have antimicrobial action against a spectrum of bacteria and fungi. Because very little quantitative information exists on the microbiota of honey, the aim of this study was to quantify the Actinobacteria, Firmicutes, and Gammaproteobacteria groups in samples of honeydew honey and blossom honey from six regions in the Czech Republic, using quantitative realtime PCR analysis with specific primers based on the 16S rRNA gene. Gammaproteobacteria and Firmicutes were clearly the most abundant, predominating Actinobacteria in both types of honey. Most of the Firmicutes were detected in samples from South Bohemia (mean gene copies per 1 g honey 5,6 to 105) and Ústí nad Labem Region (3,7 to 105), which contained the lowest number of Gammaproteobacteria (15,5 to 103). The Actinobacteria were prevalent in samples from Plzeň (4,3 to 103) and Central Bohemia (5,4 to 103), where conversely the Firmicutes w

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Agriculturae Bohemica

ISSN

1211-3174

e-ISSN

—

Svazek periodika

49

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

192-200

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85054367824