In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F19%3A00506137" target="_blank" >RIV/67985904:_____/19:00506137 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60460709:41210/19:80268 RIV/00027014:_____/19:N0000077
Výsledek na webu
<a href="https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1490-y" target="_blank" >https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1490-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12866-019-1490-y" target="_blank" >10.1186/s12866-019-1490-y</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time
Popis výsledku v původním jazyce
BackgroundKnowledge of microbiota composition, persistence, and transmission as well as the overall function of the bacterial community is important and may be linked to honey bee health. This study aimed to investigate the inter-individual variation in the gut microbiota in honey bee larvae and pupae.ResultsIndividual larvae differed in the composition of major bacterial groups. In the majority of 5th instar bees, Firmicutes showed predominance (70%), however, after larval defecation and during pupation, the abundance decreased to 40%, in favour of Gammaproteobacteria. The 5th instar larvae hosted significantly more (P<0.001) Firmicutes than black pupae. Power calculations revealed that 11 and 18 replicate-individuals, respectively, were required for the detection of significant differences (P<0.05) in the Bacteroidetes and Firmicutes abundance between stages, while higher numbers of replicates were required for Actinobacteria (478 replicates) and Gammaproteobacteria (111 replicates).ConclusionsAlthough sample processing and extraction protocols may have had a significant influence, sampling is very important for studying the bee microbiome, and the importance of the number of individuals pooled in samples used for microbiome studies should not be underestimated.
Název v anglickém jazyce
In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time
Popis výsledku anglicky
BackgroundKnowledge of microbiota composition, persistence, and transmission as well as the overall function of the bacterial community is important and may be linked to honey bee health. This study aimed to investigate the inter-individual variation in the gut microbiota in honey bee larvae and pupae.ResultsIndividual larvae differed in the composition of major bacterial groups. In the majority of 5th instar bees, Firmicutes showed predominance (70%), however, after larval defecation and during pupation, the abundance decreased to 40%, in favour of Gammaproteobacteria. The 5th instar larvae hosted significantly more (P<0.001) Firmicutes than black pupae. Power calculations revealed that 11 and 18 replicate-individuals, respectively, were required for the detection of significant differences (P<0.05) in the Bacteroidetes and Firmicutes abundance between stages, while higher numbers of replicates were required for Actinobacteria (478 replicates) and Gammaproteobacteria (111 replicates).ConclusionsAlthough sample processing and extraction protocols may have had a significant influence, sampling is very important for studying the bee microbiome, and the importance of the number of individuals pooled in samples used for microbiome studies should not be underestimated.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/QJ1610248" target="_blank" >QJ1610248: Dlouhověkost včel a její úloha v udržitelném chovu</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
BMC Microbiology
ISSN
1471-2180
e-ISSN
—
Svazek periodika
19
Číslo periodika v rámci svazku
MAY 24
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
8
Strana od-do
110
Kód UT WoS článku
000468899800001
EID výsledku v databázi Scopus
2-s2.0-85066396372