Applicability of low-flow atmospheric pressure chemical ionization and photoionization mass spectrometry with a microfabricated nebulizer for neutral lipids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A78619" target="_blank" >RIV/60460709:41210/18:78619 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/18:00489841 RIV/00216208:11310/18:10374500

Výsledek na webu

<a href="http://dx.doi.org/10.1002/rcm.8086" target="_blank" >http://dx.doi.org/10.1002/rcm.8086</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/rcm.8086" target="_blank" >10.1002/rcm.8086</a>

Jazyk výsledku

čeština

Název v původním jazyce

Applicability of low-flow atmospheric pressure chemical ionization and photoionization mass spectrometry with a microfabricated nebulizer for neutral lipids

Popis výsledku v původním jazyce

Mass spectrometry with atmospheric pressure chemical ionization or photoionization is widely used for neutral lipids involved in many fundamental processes in living organisms. Commercial APCI and APPI sources operate at high flow rates compatible with conventional high-performance liquid chromatography. However, lipid analysis is often limited by a small amount of sample, which requires low flow rate separations like capillary or micro HPLC. Therefore, APCI and APPI suitable for microliter per minute flow rates need to be developed and applied for neutral lipids. Micro APCI or APPI source with a heated chip nebulizer was assembled and mounted on a Thermo ion trap instrument. The ion source operated in APCI, APPI or dual mode was optimized for low microliter per minute sample flow rates. The source performance was investigated for squalene, wax esters, fatty acid methyl esters, triacylglycerols, and cholesterol. The ion source behaved as a mass flow sensitive detector. Direct infusion of methyl oleat

Název v anglickém jazyce

Applicability of low-flow atmospheric pressure chemical ionization and photoionization mass spectrometry with a microfabricated nebulizer for neutral lipids

Popis výsledku anglicky

Mass spectrometry with atmospheric pressure chemical ionization or photoionization is widely used for neutral lipids involved in many fundamental processes in living organisms. Commercial APCI and APPI sources operate at high flow rates compatible with conventional high-performance liquid chromatography. However, lipid analysis is often limited by a small amount of sample, which requires low flow rate separations like capillary or micro HPLC. Therefore, APCI and APPI suitable for microliter per minute flow rates need to be developed and applied for neutral lipids. Micro APCI or APPI source with a heated chip nebulizer was assembled and mounted on a Thermo ion trap instrument. The ion source operated in APCI, APPI or dual mode was optimized for low microliter per minute sample flow rates. The source performance was investigated for squalene, wax esters, fatty acid methyl esters, triacylglycerols, and cholesterol. The ion source behaved as a mass flow sensitive detector. Direct infusion of methyl oleat

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA16-01639S" target="_blank" >GA16-01639S: Mikro- a nanoprůtokové ionizace za atmosferického tlaku pro bioanalytickou hmotnostní spektrometrii</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RAPID COMMUNICATIONS IN MASS SPECTROMETRY

ISSN

0951-4198

e-ISSN

1097-0231

Svazek periodika

32

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

639-648

Kód UT WoS článku

000428526800006

EID výsledku v databázi Scopus

2-s2.0-85044509116