Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3A77466" target="_blank" >RIV/60460709:41210/19:77466 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/19:00501628 RIV/00216208:11310/19:10394485

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0141813018354448?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0141813018354448?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2018.11.161" target="_blank" >10.1016/j.ijbiomac.2018.11.161</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos

Popis výsledku v původním jazyce

etraspanins are multifunctional molecules located in specific microdomains on the plasma membrane. Thanks to their ability to form networks with other proteins they can participate in many cellular functions. Tetraspanins are part of the interactive network in gametes. However, their precise role in fertilization is not yet clear. The aim of this study was to compare the localization of CD9 and CD81 tetraspanins during oocyte maturation and early development of the embryos in bovine and porcine model. CD9 was detected on the oocyte plasma membrane and vesicles in the perivitelline space of bovine oocytes and embryos. We suggest that CD9 could be a component involved in transzonal projections. Based on the results of in vitro fertilization assay, CD9 and CD81 seem to be part of a more complex fusion network on the plasma membrane of bovine oocytes. On the other hand, both tetraspanins showed a clustered expression pattern on the plasma membrane and inner margin of zona pellucida in porcine oocytes and

Název v anglickém jazyce

Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos

Popis výsledku anglicky

etraspanins are multifunctional molecules located in specific microdomains on the plasma membrane. Thanks to their ability to form networks with other proteins they can participate in many cellular functions. Tetraspanins are part of the interactive network in gametes. However, their precise role in fertilization is not yet clear. The aim of this study was to compare the localization of CD9 and CD81 tetraspanins during oocyte maturation and early development of the embryos in bovine and porcine model. CD9 was detected on the oocyte plasma membrane and vesicles in the perivitelline space of bovine oocytes and embryos. We suggest that CD9 could be a component involved in transzonal projections. Based on the results of in vitro fertilization assay, CD9 and CD81 seem to be part of a more complex fusion network on the plasma membrane of bovine oocytes. On the other hand, both tetraspanins showed a clustered expression pattern on the plasma membrane and inner margin of zona pellucida in porcine oocytes and

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

<a href="/cs/project/LTC18059" target="_blank" >LTC18059: Kultivační systém vhodný pro dlouhodobou kultivaci lidských folikulárních buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ISSN

0141-8130

e-ISSN

0141-8130

Svazek periodika

123

Číslo periodika v rámci svazku

N

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

931-938

Kód UT WoS článku

000456760100103

EID výsledku v databázi Scopus

2-s2.0-85056846481