Estimation of genotyping errors of STR markers in dogs and wolves

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3A80820" target="_blank" >RIV/60460709:41210/19:80820 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1875176819300800?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1875176819300800?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fsigss.2019.11.014" target="_blank" >10.1016/j.fsigss.2019.11.014</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Estimation of genotyping errors of STR markers in dogs and wolves

Popis výsledku v původním jazyce

This article will discuss the estimation of genotyping errors for Mini-DogFiler panel A and B in group of wolfs (n = 39) where the source material for DNA isolation were faeces and control group of dogs (n = 170), represented by Czechoslovakian wolfdog breed (CSW), where DNA was obtained from buccal swabs. DNA samples isolated from buccal swabs had a very low frequency of genotyping error estimated as mean error rate per allele (ea = 0,09%), mean error per locus (el = 0,18%) and error rate per multilocus genotype (eobs = 2,17%). Completely different situation was observed for DNA samples isolated from faeces and affirms how complicated is to get reliable results from such material. Total performance was very poor where only 7 out of 39 samples replicated (about 18%) at least 2 times out of 4 replicates. In such case would be eobs = 86,5%. For this type of samples is typical very fragmented DNA with low concentration. This fact leads to the occurrence of many of negative effects like an allelic dropou

Název v anglickém jazyce

Estimation of genotyping errors of STR markers in dogs and wolves

Popis výsledku anglicky

This article will discuss the estimation of genotyping errors for Mini-DogFiler panel A and B in group of wolfs (n = 39) where the source material for DNA isolation were faeces and control group of dogs (n = 170), represented by Czechoslovakian wolfdog breed (CSW), where DNA was obtained from buccal swabs. DNA samples isolated from buccal swabs had a very low frequency of genotyping error estimated as mean error rate per allele (ea = 0,09%), mean error per locus (el = 0,18%) and error rate per multilocus genotype (eobs = 2,17%). Completely different situation was observed for DNA samples isolated from faeces and affirms how complicated is to get reliable results from such material. Total performance was very poor where only 7 out of 39 samples replicated (about 18%) at least 2 times out of 4 replicates. In such case would be eobs = 86,5%. For this type of samples is typical very fragmented DNA with low concentration. This fact leads to the occurrence of many of negative effects like an allelic dropou

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30501 - Forensic science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Forensic Science International: Genetics Supplement Series

ISSN

1875-1768

e-ISSN

1875-175X

Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

901-902

Kód UT WoS článku

000508217000344

EID výsledku v databázi Scopus

2-s2.0-85076235622