Does the in vitro egg hatch test predict the failure of benzimidazole treatment in Haemonchus contortus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F21%3A85755" target="_blank" >RIV/60460709:41210/21:85755 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375488/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375488/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1051/parasite/2021059" target="_blank" >10.1051/parasite/2021059</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Does the in vitro egg hatch test predict the failure of benzimidazole treatment in Haemonchus contortus

Popis výsledku v původním jazyce

Considerable research has been directed towards optimising in vitro tests that can diagnose resistance in pre-parasitic stages of parasites. The objective of this study was to compare the in vivo faecal egg count reduction test (FECRT), the in vitro egg hatch test (EHT), and the molecular determination of the frequency of a codon 200 allele of beta-tubulin isotype 1 associated with benzimidazole resistance in larval stages of Haemonchus contortus obtained from infected goats. Animals were infected with composite infective doses representing 10, 20, 30, 40, 60, and 80% resistant alleles. Faecal samples for the EHT were collected on 28, 33, and 35 days post-infection. The results of the in vivo FECRT indicated that albendazole treatment reduced infections consisting of composite doses of 10, 20, 30, 40, 60, and 80% larvae of the resistant isolate by 91,3, 78,0, 63,3, 48,4, 36,5, and 41,4%, respectively. The drug concentration at which 50% of the eggs were prevented from developing hatching larvae (ED50

Název v anglickém jazyce

Does the in vitro egg hatch test predict the failure of benzimidazole treatment in Haemonchus contortus

Popis výsledku anglicky

Considerable research has been directed towards optimising in vitro tests that can diagnose resistance in pre-parasitic stages of parasites. The objective of this study was to compare the in vivo faecal egg count reduction test (FECRT), the in vitro egg hatch test (EHT), and the molecular determination of the frequency of a codon 200 allele of beta-tubulin isotype 1 associated with benzimidazole resistance in larval stages of Haemonchus contortus obtained from infected goats. Animals were infected with composite infective doses representing 10, 20, 30, 40, 60, and 80% resistant alleles. Faecal samples for the EHT were collected on 28, 33, and 35 days post-infection. The results of the in vivo FECRT indicated that albendazole treatment reduced infections consisting of composite doses of 10, 20, 30, 40, 60, and 80% larvae of the resistant isolate by 91,3, 78,0, 63,3, 48,4, 36,5, and 41,4%, respectively. The drug concentration at which 50% of the eggs were prevented from developing hatching larvae (ED50

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasite - Journal de la Societe Francaise de Parasitologie

ISSN

1252-607X

e-ISSN

1776-1042

Svazek periodika

28

Číslo periodika v rámci svazku

August 2021

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

6

Strana od-do

0-0

Kód UT WoS článku

000686297600001

EID výsledku v databázi Scopus

2-s2.0-85113488802