Glucose non-fermenting bifidobacteria are frequent in the hen caeca.

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Glucose non-fermenting bifidobacteria are frequent in the hen caeca.

Popis výsledku v původním jazyce

Two media were evaluated to determine their electivity and selectivity for bifidobacteria in the hen caeca samples : Wilkins-Chalgren agar (MW; Oxoid) modified by the addition of acetic acid (1 ml/l) and TPY agar (MTPY; ADSA) with acetic acid (1 ml/l) and mupirocin (100 mg/l). The colonies arising on the plates inoculated with caecal samples were Gram stained, acreeneed for the presence of fructose-6-phosphate phosphoketolase activity, and tested for fermentation patterns using ANAEROtest (Lachema, Czech Republic) and API 50CHL (Biomérieux, France) kits. MTPY agar was both more elective and more selective than MW agar. Caecal bifidobacteria counts were higher than using MTPY agar. Higher electivity of MTPY agar was due to soya peptone with high carbohydrate content. Most of isolates from this medium were glucose negative and rafinose positive.

Název v anglickém jazyce

Glucose non-fermenting bifidobacteria are frequent in the hen caeca.

Popis výsledku anglicky

Two media were evaluated to determine their electivity and selectivity for bifidobacteria in the hen caeca samples : Wilkins-Chalgren agar (MW; Oxoid) modified by the addition of acetic acid (1 ml/l) and TPY agar (MTPY; ADSA) with acetic acid (1 ml/l) and mupirocin (100 mg/l). The colonies arising on the plates inoculated with caecal samples were Gram stained, acreeneed for the presence of fructose-6-phosphate phosphoketolase activity, and tested for fermentation patterns using ANAEROtest (Lachema, Czech Republic) and API 50CHL (Biomérieux, France) kits. MTPY agar was both more elective and more selective than MW agar. Caecal bifidobacteria counts were higher than using MTPY agar. Higher electivity of MTPY agar was due to soya peptone with high carbohydrate content. Most of isolates from this medium were glucose negative and rafinose positive.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sixth Symposium on Lactic Acid Bacteria, Book of Abstracts

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

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Název nakladatele

Netherlands Institute for Microbiology, The Netherlands

Místo vydání

Veldhoven, The Netherlands

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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