Somatic embryogenesis of the hybrid Abies cilicica * Abies cephalonica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F11%3A51524" target="_blank" >RIV/60460709:41320/11:51524 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Somatic embryogenesis of the hybrid Abies cilicica * Abies cephalonica

Popis výsledku v původním jazyce

The interspecific hybrid fir Abies cilicica * Abies cephalonica is based on native species Abies cephalonica (Loud.) and Abies cilicica (Ant. et Kotschy) Carr. Many variants of nutrient media have been tested. The medium BAP at a concentration of 1 mg l?1, 1,000 mg l?1 myo-inositol, 500 mg l?1 glutamine, and 1,000 mg l?1 casein was found to be the most suitable initiation medium for somatic embryogenesis of this hybrid. Embryogenic suspensor mass was produced from immature and mature primary explants. The initiation frequency of ESM was dependent on the composition of nutrient medium, plant growth regulators, and time of seeds collection. The ESM initiation from immature seeds was higher in comparison with mature zygotic embryos, but the results were not significantly different. As the most suitable maturation medium was evaluated the medium based on Murashige and Skoog medium which contained 10 mg l?1 abscisic acid, 100 g l?1 PEG and 40 g l?1 maltose (MM 2 medium).

Název v anglickém jazyce

Somatic embryogenesis of the hybrid Abies cilicica * Abies cephalonica

Popis výsledku anglicky

The interspecific hybrid fir Abies cilicica * Abies cephalonica is based on native species Abies cephalonica (Loud.) and Abies cilicica (Ant. et Kotschy) Carr. Many variants of nutrient media have been tested. The medium BAP at a concentration of 1 mg l?1, 1,000 mg l?1 myo-inositol, 500 mg l?1 glutamine, and 1,000 mg l?1 casein was found to be the most suitable initiation medium for somatic embryogenesis of this hybrid. Embryogenic suspensor mass was produced from immature and mature primary explants. The initiation frequency of ESM was dependent on the composition of nutrient medium, plant growth regulators, and time of seeds collection. The ESM initiation from immature seeds was higher in comparison with mature zygotic embryos, but the results were not significantly different. As the most suitable maturation medium was evaluated the medium based on Murashige and Skoog medium which contained 10 mg l?1 abscisic acid, 100 g l?1 PEG and 40 g l?1 maltose (MM 2 medium).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

<a href="/cs/project/ME%20914" target="_blank" >ME 914: Šlechtění jedle pro lesní hospodářství a produkci vánočních stromků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Forest Science

ISSN

1212-4834

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

401-408

Kód UT WoS článku

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EID výsledku v databázi Scopus

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