The role of auxins in somatic embryogenesis of Abies alba

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00367372" target="_blank" >RIV/61389030:_____/11:00367372 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2478/s11535-011-0035-7" target="_blank" >http://dx.doi.org/10.2478/s11535-011-0035-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11535-011-0035-7" target="_blank" >10.2478/s11535-011-0035-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of auxins in somatic embryogenesis of Abies alba

Popis výsledku v původním jazyce

We report the effects of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the auxin inhibitor p-chlorophenoxyisobutyric acid (PCIB) on the endogenous level of the auxin indole-3-acetic acid (IAA) and on the anatomical composition of early somatic embryos of Abies alba (European silver fir). The embryogenic suspensor mass (ESM) of Abies alba proliferated on a medium supplemented by 2,4-D as well as on an auxin-free medium. The endogenous level of IAA was significantly higher in the ESM cultivated on a medium supplemented by 2,4-D. The decrease in the endogenous level of IAA in the first week of maturation is one of the most important stimuli responsible for the subsequent development of embryos. Suppression of IAA synthesis by an auxin inhibitor did not stimulate the development of embryos. The maturation of somatic embryos from the globular to the cotyledonary stage occurs when the concentration of endogenous auxin in the ESM (including the embryos) increases.

Název v anglickém jazyce

The role of auxins in somatic embryogenesis of Abies alba

Popis výsledku anglicky

We report the effects of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the auxin inhibitor p-chlorophenoxyisobutyric acid (PCIB) on the endogenous level of the auxin indole-3-acetic acid (IAA) and on the anatomical composition of early somatic embryos of Abies alba (European silver fir). The embryogenic suspensor mass (ESM) of Abies alba proliferated on a medium supplemented by 2,4-D as well as on an auxin-free medium. The endogenous level of IAA was significantly higher in the ESM cultivated on a medium supplemented by 2,4-D. The decrease in the endogenous level of IAA in the first week of maturation is one of the most important stimuli responsible for the subsequent development of embryos. Suppression of IAA synthesis by an auxin inhibitor did not stimulate the development of embryos. The maturation of somatic embryos from the globular to the cotyledonary stage occurs when the concentration of endogenous auxin in the ESM (including the embryos) increases.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/OC%20158" target="_blank" >OC 158: Biochemické a morfologické parametry rozhodující o kryotoleranci embryogenních kultur jehličnanů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Central European Journal of Biology

ISSN

1895-104X

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

10

Strana od-do

587-596

Kód UT WoS článku

000290803200013

EID výsledku v databázi Scopus

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