Development, characterization, and cross-amplification of 17 microsatellite markers for Filipendula vulgaris

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41330%2F19%3A80099" target="_blank" >RIV/60460709:41330/19:80099 - isvavai.cz</a>

Výsledek na webu

<a href="https://bsapubs.onlinelibrary.wiley.com/doi/full/10.1002/aps3.11307" target="_blank" >https://bsapubs.onlinelibrary.wiley.com/doi/full/10.1002/aps3.11307</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/aps3.11307" target="_blank" >10.1002/aps3.11307</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development, characterization, and cross-amplification of 17 microsatellite markers for Filipendula vulgaris

Popis výsledku v původním jazyce

Seventeen new polymorphic microsatellite markers were developed for F. vulgaris using the Illumina MiSeq platform. Polymorphism of the 17 loci was tested in three populations. We identified a total of 203 alleles, ranging from four to 19 per locus, with levels of observed and expected heterozygosity ranging from 0,267 to 1,000 and 0,461 to 0,899, respectively. Primers were also tested for cross-amplification in three related species. Seven loci successfully cross amplified in F. camtschatica and F. ulmaria, whereas we detected positive cross-amplification in only three loci in Geum urbanum. The newly developed microsatellite primers will serve as useful genetic tools for further population genetic studies on F. vulgaris and related species.

Název v anglickém jazyce

Development, characterization, and cross-amplification of 17 microsatellite markers for Filipendula vulgaris

Popis výsledku anglicky

Seventeen new polymorphic microsatellite markers were developed for F. vulgaris using the Illumina MiSeq platform. Polymorphism of the 17 loci was tested in three populations. We identified a total of 203 alleles, ranging from four to 19 per locus, with levels of observed and expected heterozygosity ranging from 0,267 to 1,000 and 0,461 to 0,899, respectively. Primers were also tested for cross-amplification in three related species. Seven loci successfully cross amplified in F. camtschatica and F. ulmaria, whereas we detected positive cross-amplification in only three loci in Geum urbanum. The newly developed microsatellite primers will serve as useful genetic tools for further population genetic studies on F. vulgaris and related species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applications in Plant Sciences 

ISSN

2168-0450

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

1-4

Kód UT WoS článku

000501117000001

EID výsledku v databázi Scopus

2-s2.0-85076748781