A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F13%3A60548" target="_blank" >RIV/60460709:41340/13:60548 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION

Popis výsledku v původním jazyce

Yacon (Smallanthus sonchifolius) is an Andean crop which is very regarded for its benefits for people suffering from diabetes or various digestive or renal disorders. Because no specific Smallanthus sonchifolius identification DNA markers are still knownthe paper demonstrates ITS regions to be able to detect and differentiate among yacon species and the potential for specific food authentification purposes is reported, too. The newly sequenced ITS of yacon accessions originated in Peru, Ecuador and Bolivia analyse provide the unique sequence site that differs from all of the other yacon species and is recognized by DraIII restriction endonuclease. Restriction cleavadge of the PCR amplified ITSs of the twenty-eight yacon accessions was performed and inall cases the recognition site was confirmed as a typical for Smallanthus sonchifolius . Based on the nucleotide specifity of Smallanthus sonchifolius, ITS sequence the PCR method combined with the restriction clevadge protocol wa

Název v anglickém jazyce

A REVISED ITS NUCLEOTIDE SEQUENCE GIVES A SPECIFITY FOR Smallanthus sonchifolius (POEPP. AND ENDL.) AND ITS PRODUCTS IDENTIFICATION

Popis výsledku anglicky

Yacon (Smallanthus sonchifolius) is an Andean crop which is very regarded for its benefits for people suffering from diabetes or various digestive or renal disorders. Because no specific Smallanthus sonchifolius identification DNA markers are still knownthe paper demonstrates ITS regions to be able to detect and differentiate among yacon species and the potential for specific food authentification purposes is reported, too. The newly sequenced ITS of yacon accessions originated in Peru, Ecuador and Bolivia analyse provide the unique sequence site that differs from all of the other yacon species and is recognized by DraIII restriction endonuclease. Restriction cleavadge of the PCR amplified ITSs of the twenty-eight yacon accessions was performed and inall cases the recognition site was confirmed as a typical for Smallanthus sonchifolius . Based on the nucleotide specifity of Smallanthus sonchifolius, ITS sequence the PCR method combined with the restriction clevadge protocol wa

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

GENETIKA-BELGRADE

ISSN

0534-0012

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

217-226

Kód UT WoS článku

000320985900021

EID výsledku v databázi Scopus

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