In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F14%3A63702" target="_blank" >RIV/60460709:41340/14:63702 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Popis výsledku v původním jazyce

This study was aimed to optimize in vitro propagation of Drosera intermedia for commercial and conservation purposes. The effect of concentration of MS nutrients (? MS, 1 MS, 1 MS and, MS), various pH (3.7-7.7), sucrose concentration (10-40 g l-1) and cytokinins (0.1-3 mg l-1), namely BA (N6-benzyladenine), kinetin and zeatin were evaluated. After 60 days of shoot cultivation, growth and developmental characteristics (plant height, number of shoots per explant, diameter of rosette, number of roots per explant, length of roots) were recorded. No significant differences were found for various levels of pH and sucrose. On the contrary, plant height was negatively influenced by an increase of nutrients in the medium. The plants on 1/8 MS medium were significantly taller, and displayed higher proliferation capacity compared with those cultivated on full-strength MS medium. Shoot multiplication and growth was suppressed by supplementation of BA and kinetin, regardless of concentration used.

Název v anglickém jazyce

In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Popis výsledku anglicky

This study was aimed to optimize in vitro propagation of Drosera intermedia for commercial and conservation purposes. The effect of concentration of MS nutrients (? MS, 1 MS, 1 MS and, MS), various pH (3.7-7.7), sucrose concentration (10-40 g l-1) and cytokinins (0.1-3 mg l-1), namely BA (N6-benzyladenine), kinetin and zeatin were evaluated. After 60 days of shoot cultivation, growth and developmental characteristics (plant height, number of shoots per explant, diameter of rosette, number of roots per explant, length of roots) were recorded. No significant differences were found for various levels of pH and sucrose. On the contrary, plant height was negatively influenced by an increase of nutrients in the medium. The plants on 1/8 MS medium were significantly taller, and displayed higher proliferation capacity compared with those cultivated on full-strength MS medium. Shoot multiplication and growth was suppressed by supplementation of BA and kinetin, regardless of concentration used.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Emirates Journal of Food and Agriculture

ISSN

2079-052X

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

558-564

Kód UT WoS článku

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EID výsledku v databázi Scopus

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