IRAP VARIABILITY IN Prunus domestica (L.) Borkh BASED ON Cassandra RETROTRANSPOSON POLYMORPHISM

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F17%3A75282" target="_blank" >RIV/60460709:41340/17:75282 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2298/GENSR1703989S" target="_blank" >http://dx.doi.org/10.2298/GENSR1703989S</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2298/GENSR1703989S" target="_blank" >10.2298/GENSR1703989S</a>

Jazyk výsledku

angličtina

Název v původním jazyce

IRAP VARIABILITY IN Prunus domestica (L.) Borkh BASED ON Cassandra RETROTRANSPOSON POLYMORPHISM

Popis výsledku v původním jazyce

Plum species are reported to possess a wide genomic variability and that is why DNA markers are still actual in the characterization of its germlasm. In this study, twenty-three genotypes of European plums were assessed for the amplified length based polymorphism among the retrotransposon Cassandra insertions in their genomes. The obtained insertional polymorphism caused by the activity of Cassandra showed regional and pedigree differences in the analysed accessions of European plums. Two primers were used in analysis. The first resulted in the amplification in 203 amplicons and the 86,6 % polymorphism. Two unique fragments were obtained for the Torysa and Podolínec varieties using this primer. The second primer resulted in the amplification in 267 amplicons and 74,2 % polymorphism. Four unique fragments were obtained for the plum varieties Švestka domácí, Čačanská ranná and Elena. Hierarchical cluster analysis divided the analysed accessions into the four main clusters. To show the length polymorphi

Název v anglickém jazyce

IRAP VARIABILITY IN Prunus domestica (L.) Borkh BASED ON Cassandra RETROTRANSPOSON POLYMORPHISM

Popis výsledku anglicky

Plum species are reported to possess a wide genomic variability and that is why DNA markers are still actual in the characterization of its germlasm. In this study, twenty-three genotypes of European plums were assessed for the amplified length based polymorphism among the retrotransposon Cassandra insertions in their genomes. The obtained insertional polymorphism caused by the activity of Cassandra showed regional and pedigree differences in the analysed accessions of European plums. Two primers were used in analysis. The first resulted in the amplification in 203 amplicons and the 86,6 % polymorphism. Two unique fragments were obtained for the Torysa and Podolínec varieties using this primer. The second primer resulted in the amplification in 267 amplicons and 74,2 % polymorphism. Four unique fragments were obtained for the plum varieties Švestka domácí, Čačanská ranná and Elena. Hierarchical cluster analysis divided the analysed accessions into the four main clusters. To show the length polymorphi

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

GENETIKA-BELGRADE

ISSN

0534-0012

e-ISSN

1820-6069

Svazek periodika

49

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

12

Strana od-do

989-1000

Kód UT WoS článku

000423604800019

EID výsledku v databázi Scopus

2-s2.0-85042104020