UTILIZATION OF CDDP MARKERS IN ANALYSIS OF GENETIC VARIABILITY OF ARACHIS HYPOGAEA L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F23%3A94631" target="_blank" >RIV/60460709:41340/23:94631 - isvavai.cz</a>

Výsledek na webu

<a href="https://office2.jmbfs.org/index.php/JMBFS/article/view/9884" target="_blank" >https://office2.jmbfs.org/index.php/JMBFS/article/view/9884</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.55251/jmbfs.9884" target="_blank" >10.55251/jmbfs.9884</a>

Jazyk výsledku

angličtina

Název v původním jazyce

UTILIZATION OF CDDP MARKERS IN ANALYSIS OF GENETIC VARIABILITY OF ARACHIS HYPOGAEA L.

Popis výsledku v původním jazyce

Arachis hypogaea L. belong botanically to annual herbs, more specifically to legumes (family Fabaceae). Cultivated from is characterized as allotetraploid specie and that has a large and genome with high level of complexity as a result of natural hybridization of wild diploid species Arachis cardenasii (nn) and Arachis batizocoi. Individual analysis that are aimed to description of the genetic similarity and variability of wild and cultivated Arachis species are substantial for the knowledge of intaspecific relationships. In this study, conserved DNA derived polymorphism technique was used to analyse genetic variability of natural genotypes of peanut. CDDP is a simple PCR based method that was proved to be efficient in the studies of plant populations polymorphism. Primers used in CDDP target conserved sequences of plant functional genes that are involved in response to abiotic and biotic stresses. Here, bulked DNA samples of twenty one natural populations were analysed by with five CDDP primer combinations. Selected primers produced a total of 260 amplicons, among which 107 (41,2%) amplicons were polymorphic. The average number of obtained amplicons per primer was about 51,8. Amplified polymorphic fragments of analysed genotypes ranged from 13 to 33 and percentage of polymorphism ranged from 35 % to 47 %. UPGMA cluster analysis was performed on the base of prepared binary matrices and obtained clustering has grouped the analyzed peanut populations into five major groups with further subgroups. The results of CCDP fingerprinting shown, that this marker technique provide sufficient distinguishing of a wild population of Arachis hypogaea L.. Analysed genotypes of peanut were separated into different groups based on genetic diversity, however, the level of polymorphisms that detected used markers among populations of Arachis hypogaea L. was low.

Název v anglickém jazyce

UTILIZATION OF CDDP MARKERS IN ANALYSIS OF GENETIC VARIABILITY OF ARACHIS HYPOGAEA L.

Popis výsledku anglicky

Arachis hypogaea L. belong botanically to annual herbs, more specifically to legumes (family Fabaceae). Cultivated from is characterized as allotetraploid specie and that has a large and genome with high level of complexity as a result of natural hybridization of wild diploid species Arachis cardenasii (nn) and Arachis batizocoi. Individual analysis that are aimed to description of the genetic similarity and variability of wild and cultivated Arachis species are substantial for the knowledge of intaspecific relationships. In this study, conserved DNA derived polymorphism technique was used to analyse genetic variability of natural genotypes of peanut. CDDP is a simple PCR based method that was proved to be efficient in the studies of plant populations polymorphism. Primers used in CDDP target conserved sequences of plant functional genes that are involved in response to abiotic and biotic stresses. Here, bulked DNA samples of twenty one natural populations were analysed by with five CDDP primer combinations. Selected primers produced a total of 260 amplicons, among which 107 (41,2%) amplicons were polymorphic. The average number of obtained amplicons per primer was about 51,8. Amplified polymorphic fragments of analysed genotypes ranged from 13 to 33 and percentage of polymorphism ranged from 35 % to 47 %. UPGMA cluster analysis was performed on the base of prepared binary matrices and obtained clustering has grouped the analyzed peanut populations into five major groups with further subgroups. The results of CCDP fingerprinting shown, that this marker technique provide sufficient distinguishing of a wild population of Arachis hypogaea L.. Analysed genotypes of peanut were separated into different groups based on genetic diversity, however, the level of polymorphisms that detected used markers among populations of Arachis hypogaea L. was low.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40101 - Agriculture

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Biotechnology and Food Sciences

ISSN

1338-5178

e-ISSN

1338-5178

Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000960532200001

EID výsledku v databázi Scopus

2-s2.0-85169050920