Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markers
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F23%3A96612" target="_blank" >RIV/60460709:41340/23:96612 - isvavai.cz</a>
Výsledek na webu
<a href="https://pse.agriculturejournals.cz/artkey/pse-202312-0006_polymorphism-of-bolivian-accessions-of-arachis-hypogaea-l-revealed-by-allergen-coding-dna-markers.php" target="_blank" >https://pse.agriculturejournals.cz/artkey/pse-202312-0006_polymorphism-of-bolivian-accessions-of-arachis-hypogaea-l-revealed-by-allergen-coding-dna-markers.php</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/306/2023-PSE" target="_blank" >10.17221/306/2023-PSE</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markers
Popis výsledku v původním jazyce
Arachis hypogaea L. is an annual legume that is one of the most consumed plant species. On the other hand, it belongs to one of the most monitored clinically important allergens worldwide. The polymorphism of this species based on allergen coding genes could be useful in its characterisation, but previously, no allergen-based marker techniques have been developed for peanuts. A new type of DNA-based markers of coding regions were used to analyse the variability of 21 peanut accessions – BBAP (Bet v1 based amplicon polymorphism), PBAP (profilin based amplicon polymorphism), and VBAP (vicilin based amplicon polymorphism). All of the used technique provided polymorphic fingerprints and distinguished the analysed peanut accessions. The effectivity of these techniques corresponds to the presence of the allergen homologous sequences that are a part of the A. hypogaea genome. VBAP was the most effective in distinguishing the analysed peanut accessions when compared to the results of BBAP and PBAB. For BBAP, two of the analysed accessions provided the same fingerprinting pattern. The ability of the used markers to detect polymorphisms was comparable, with an average polymorphism information content (PIC) value of 0.47.
Název v anglickém jazyce
Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markers
Popis výsledku anglicky
Arachis hypogaea L. is an annual legume that is one of the most consumed plant species. On the other hand, it belongs to one of the most monitored clinically important allergens worldwide. The polymorphism of this species based on allergen coding genes could be useful in its characterisation, but previously, no allergen-based marker techniques have been developed for peanuts. A new type of DNA-based markers of coding regions were used to analyse the variability of 21 peanut accessions – BBAP (Bet v1 based amplicon polymorphism), PBAP (profilin based amplicon polymorphism), and VBAP (vicilin based amplicon polymorphism). All of the used technique provided polymorphic fingerprints and distinguished the analysed peanut accessions. The effectivity of these techniques corresponds to the presence of the allergen homologous sequences that are a part of the A. hypogaea genome. VBAP was the most effective in distinguishing the analysed peanut accessions when compared to the results of BBAP and PBAB. For BBAP, two of the analysed accessions provided the same fingerprinting pattern. The ability of the used markers to detect polymorphisms was comparable, with an average polymorphism information content (PIC) value of 0.47.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40101 - Agriculture
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plant, Soil and Environment
ISSN
1214-1178
e-ISSN
1214-1178
Svazek periodika
69
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
13
Strana od-do
615-627
Kód UT WoS článku
001127081800001
EID výsledku v databázi Scopus
2-s2.0-85180450786