Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F23%3A96612" target="_blank" >RIV/60460709:41340/23:96612 - isvavai.cz</a>

Výsledek na webu

<a href="https://pse.agriculturejournals.cz/artkey/pse-202312-0006_polymorphism-of-bolivian-accessions-of-arachis-hypogaea-l-revealed-by-allergen-coding-dna-markers.php" target="_blank" >https://pse.agriculturejournals.cz/artkey/pse-202312-0006_polymorphism-of-bolivian-accessions-of-arachis-hypogaea-l-revealed-by-allergen-coding-dna-markers.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/306/2023-PSE" target="_blank" >10.17221/306/2023-PSE</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markers

Popis výsledku v původním jazyce

Arachis hypogaea L. is an annual legume that is one of the most consumed plant species. On the other hand, it belongs to one of the most monitored clinically important allergens worldwide. The polymorphism of this species based on allergen coding genes could be useful in its characterisation, but previously, no allergen-based marker techniques have been developed for peanuts. A new type of DNA-based markers of coding regions were used to analyse the variability of 21 peanut accessions – BBAP (Bet v1 based amplicon polymorphism), PBAP (profilin based amplicon polymorphism), and VBAP (vicilin based amplicon polymorphism). All of the used technique provided polymorphic fingerprints and distinguished the analysed peanut accessions. The effectivity of these techniques corresponds to the presence of the allergen homologous sequences that are a part of the A. hypogaea genome. VBAP was the most effective in distinguishing the analysed peanut accessions when compared to the results of BBAP and PBAB. For BBAP, two of the analysed accessions provided the same fingerprinting pattern. The ability of the used markers to detect polymorphisms was comparable, with an average polymorphism information content (PIC) value of 0.47.

Název v anglickém jazyce

Polymorphism of Bolivian accessions of Arachis hypogaea L. revealed by allergen coding DNA markers

Popis výsledku anglicky

Arachis hypogaea L. is an annual legume that is one of the most consumed plant species. On the other hand, it belongs to one of the most monitored clinically important allergens worldwide. The polymorphism of this species based on allergen coding genes could be useful in its characterisation, but previously, no allergen-based marker techniques have been developed for peanuts. A new type of DNA-based markers of coding regions were used to analyse the variability of 21 peanut accessions – BBAP (Bet v1 based amplicon polymorphism), PBAP (profilin based amplicon polymorphism), and VBAP (vicilin based amplicon polymorphism). All of the used technique provided polymorphic fingerprints and distinguished the analysed peanut accessions. The effectivity of these techniques corresponds to the presence of the allergen homologous sequences that are a part of the A. hypogaea genome. VBAP was the most effective in distinguishing the analysed peanut accessions when compared to the results of BBAP and PBAB. For BBAP, two of the analysed accessions provided the same fingerprinting pattern. The ability of the used markers to detect polymorphisms was comparable, with an average polymorphism information content (PIC) value of 0.47.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40101 - Agriculture

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant, Soil and Environment

ISSN

1214-1178

e-ISSN

1214-1178

Svazek periodika

69

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

13

Strana od-do

615-627

Kód UT WoS článku

001127081800001

EID výsledku v databázi Scopus

2-s2.0-85180450786