Genetic diversity and population structure analysis of avocados (<i>Persea americana</i> Mill.) from Southern Ethiopia and Guatemala using polymorphic simple sequence repeat (SSR) markers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F24%3A100909" target="_blank" >RIV/60460709:41340/24:100909 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s10722-023-01831-1" target="_blank" >https://doi.org/10.1007/s10722-023-01831-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10722-023-01831-1" target="_blank" >10.1007/s10722-023-01831-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genetic diversity and population structure analysis of avocados (<i>Persea americana</i> Mill.) from Southern Ethiopia and Guatemala using polymorphic simple sequence repeat (SSR) markers

Popis výsledku v původním jazyce

Avocado (Persea americana Mill.) is a fruit tree species that grows in different regions of the world. The edible fruit and other parts of the avocado tree have immense nutritional, oil-bearing, and medicinal value. The plant is native to Guatemala but was introduced to Ethiopia by missionaries in 1939. In southern Ethiopia, where coffee is the main cash crop, it is used as a shade for plants. However, in the past 25 years, many farmers and urban dwellers have begun to use avocados as a valuable source of income. Hence, there is a need for quality avocado trees for commercial purposes. Although there is conjecture about the wide variation in avocados in Ethiopia, the diversity and population structure of this plant has not been described. This study aims to fill this gap by comparing avocado germplasm from Guatemala and Ethiopia. A sample of 298 avocados was collected from three and four populations in Guatemala and Ethiopia, respectively. Population diversity and structure were assessed using 12 simple sequence repeat (SSR) markers. The SSR marker profile detected 112 alleles, with an average of 10.21 per locus and an average PIC value of 0.84. Genetic diversity was high, with an average of 10.2 alleles per locus, a mean Shannon index value of 1.65, and observed and expected heterozygosity of 0.51 and 0.71, respectively. The expected heterozygosity varied between populations and was highest in West Guatemala (0.85) and lowest in Gamo (0.55). An analysis of molecular variance showed that 18% of the genetic variation was between the continents and only 2% among the populations. The pairwise genetic differentiation coefficient (F-ST) indicated high differentiation between populations. The phylogenetic tree, structure, discriminant analysis of the main components, and Nei's genetic distance analysis confirmed three groups of avocados. The Wondo Genet population was more genetically diverse than the populations from the Sidama, Wolaita, and Gamo regions. Furthermore, it revealed little genetic exchange with these populations, unlike the Guatemalan avocado, indicating the presence of unique alleles among avocados in Ethiopia. The landraces from Sidama, Wolaita, and Gamo appeared to have experienced historical drift events.

Název v anglickém jazyce

Genetic diversity and population structure analysis of avocados (<i>Persea americana</i> Mill.) from Southern Ethiopia and Guatemala using polymorphic simple sequence repeat (SSR) markers

Popis výsledku anglicky

Avocado (Persea americana Mill.) is a fruit tree species that grows in different regions of the world. The edible fruit and other parts of the avocado tree have immense nutritional, oil-bearing, and medicinal value. The plant is native to Guatemala but was introduced to Ethiopia by missionaries in 1939. In southern Ethiopia, where coffee is the main cash crop, it is used as a shade for plants. However, in the past 25 years, many farmers and urban dwellers have begun to use avocados as a valuable source of income. Hence, there is a need for quality avocado trees for commercial purposes. Although there is conjecture about the wide variation in avocados in Ethiopia, the diversity and population structure of this plant has not been described. This study aims to fill this gap by comparing avocado germplasm from Guatemala and Ethiopia. A sample of 298 avocados was collected from three and four populations in Guatemala and Ethiopia, respectively. Population diversity and structure were assessed using 12 simple sequence repeat (SSR) markers. The SSR marker profile detected 112 alleles, with an average of 10.21 per locus and an average PIC value of 0.84. Genetic diversity was high, with an average of 10.2 alleles per locus, a mean Shannon index value of 1.65, and observed and expected heterozygosity of 0.51 and 0.71, respectively. The expected heterozygosity varied between populations and was highest in West Guatemala (0.85) and lowest in Gamo (0.55). An analysis of molecular variance showed that 18% of the genetic variation was between the continents and only 2% among the populations. The pairwise genetic differentiation coefficient (F-ST) indicated high differentiation between populations. The phylogenetic tree, structure, discriminant analysis of the main components, and Nei's genetic distance analysis confirmed three groups of avocados. The Wondo Genet population was more genetically diverse than the populations from the Sidama, Wolaita, and Gamo regions. Furthermore, it revealed little genetic exchange with these populations, unlike the Guatemalan avocado, indicating the presence of unique alleles among avocados in Ethiopia. The landraces from Sidama, Wolaita, and Gamo appeared to have experienced historical drift events.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetic Resources and Crop Evolution

ISSN

0925-9864

e-ISSN

0925-9864

Svazek periodika

71

Číslo periodika v rámci svazku

OCT 2024

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

16

Strana od-do

3331-3346

Kód UT WoS článku

001152170900001

EID výsledku v databázi Scopus

2-s2.0-85182487623