Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) marker efficacy for maize hybrid identification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00173268" target="_blank" >RIV/62156489:43210/11:00173268 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) marker efficacy for maize hybrid identification

Popis výsledku v původním jazyce

Knowledge as to genetic diversity and relationships among maize hybrids is important for breeding strategies. The main aims of this study were to (1) estimate molecular genetic diversity among 30 maize hybrids by random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers; and (2) compare the genetic relatedness values obtained from these marker types. A set of 30 maize hybrids was assessed. To compare these two methods, genetic parameters were computed such as the number of polymorphic bands, average number of alleles per locus, effective number of alleles per locus, expected heterozygosity, effectiveness index of analysis and polymorphism information content (PIC).Better results were provided by SSR. The discrimination ability ofindividual markers was also determined. The SSR system provided an average PIC of 0.71 (ranging from 0.47 to 0.91) and RAPD provided an average value of 0.61 (ranging from 0.44 to 0.82). Genetic similarities (GS) were estimated using Nei

Název v anglickém jazyce

Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) marker efficacy for maize hybrid identification

Popis výsledku anglicky

Knowledge as to genetic diversity and relationships among maize hybrids is important for breeding strategies. The main aims of this study were to (1) estimate molecular genetic diversity among 30 maize hybrids by random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers; and (2) compare the genetic relatedness values obtained from these marker types. A set of 30 maize hybrids was assessed. To compare these two methods, genetic parameters were computed such as the number of polymorphic bands, average number of alleles per locus, effective number of alleles per locus, expected heterozygosity, effectiveness index of analysis and polymorphism information content (PIC).Better results were provided by SSR. The discrimination ability ofindividual markers was also determined. The SSR system provided an average PIC of 0.71 (ranging from 0.47 to 0.91) and RAPD provided an average value of 0.61 (ranging from 0.44 to 0.82). Genetic similarities (GS) were estimated using Nei

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

African Journal of Biotechnology

ISSN

1684-5315

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

24

Stát vydavatele periodika

NE - Nigerijská federativní republika

Počet stran výsledku

8

Strana od-do

4794-4801

Kód UT WoS článku

294613200002

EID výsledku v databázi Scopus

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