Exogenous applications of humic acid and salicylic acid differentially affect biomass accumulation and production of rare pharmacologically active withanolides in in vitro cultures of Withania coagulans

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F25%3A103790" target="_blank" >RIV/60460709:41340/25:103790 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.tandfonline.com/doi/full/10.1080/11263504.2025.2489989?scroll=top&needAccess=true#abstract" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/11263504.2025.2489989?scroll=top&needAccess=true#abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/11263504.2025.2489989" target="_blank" >10.1080/11263504.2025.2489989</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exogenous applications of humic acid and salicylic acid differentially affect biomass accumulation and production of rare pharmacologically active withanolides in in vitro cultures of Withania coagulans

Popis výsledku v původním jazyce

Ashwagandha (Withania coagulans) is a medicinal plant valued for its withanolide content, known for anti-cancer, anti-microbial, immunomodulatory, and hepatoprotective properties.However, due to habitat losss and overharvesting, it is now considered endangered. This study aimed to enhance biomass and withanolide production in W. coagulans callus cultures using humic acid (HA) and salicylic acid (SA) as elicitors. HA at 10 µM significantly boosted biomass (fresh weight (FW): 3.6 g/L, dry weight (DW): 267 mg/L), antioxidant enzymatic activity (peroxidase (POD) and superoxidase dismutase (SOD)) and non-enzymatic antioxidant potential, including DPPH (80.2%), ABTS (287.1 µM of Trolox equivalent antioxidant capacity (TEAC) and FRAP (548.2 µM TEAC). In contrast, SA at 60 µM negatively affected biomass and phytochemical content. HPLC analysis confirmed enhanced accumulation of withanolides and coagulanolide under HA treatment. Overall, low concentrations of HA and SA proved more effective and economically viable than high concentrations, enhancing both growth and secondary metabolite production. These findings support optimized in vitro elicitation strategies for conservation and sustainable use of W. coagulans, offering potential for commerical-scale cultivation and pharmaceutical applications. Future research may focus on understanding the molecular mechanisms behind elicitor responses and scaling up production systems to meet industrial demands.

Název v anglickém jazyce

Exogenous applications of humic acid and salicylic acid differentially affect biomass accumulation and production of rare pharmacologically active withanolides in in vitro cultures of Withania coagulans

Popis výsledku anglicky

Ashwagandha (Withania coagulans) is a medicinal plant valued for its withanolide content, known for anti-cancer, anti-microbial, immunomodulatory, and hepatoprotective properties.However, due to habitat losss and overharvesting, it is now considered endangered. This study aimed to enhance biomass and withanolide production in W. coagulans callus cultures using humic acid (HA) and salicylic acid (SA) as elicitors. HA at 10 µM significantly boosted biomass (fresh weight (FW): 3.6 g/L, dry weight (DW): 267 mg/L), antioxidant enzymatic activity (peroxidase (POD) and superoxidase dismutase (SOD)) and non-enzymatic antioxidant potential, including DPPH (80.2%), ABTS (287.1 µM of Trolox equivalent antioxidant capacity (TEAC) and FRAP (548.2 µM TEAC). In contrast, SA at 60 µM negatively affected biomass and phytochemical content. HPLC analysis confirmed enhanced accumulation of withanolides and coagulanolide under HA treatment. Overall, low concentrations of HA and SA proved more effective and economically viable than high concentrations, enhancing both growth and secondary metabolite production. These findings support optimized in vitro elicitation strategies for conservation and sustainable use of W. coagulans, offering potential for commerical-scale cultivation and pharmaceutical applications. Future research may focus on understanding the molecular mechanisms behind elicitor responses and scaling up production systems to meet industrial demands.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40101 - Agriculture

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLANT BIOSYSTEMS

ISSN

1126-3504

e-ISSN

1126-3504

Svazek periodika

159

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

15

Strana od-do

581-595

Kód UT WoS článku

001484049000001

EID výsledku v databázi Scopus

2-s2.0-105004464047