Exploring melatonin’s influence on secondary metabolism of Echinacea purpurea callus cultures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41340%2F25%3A103776" target="_blank" >RIV/60460709:41340/25:103776 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11240-025-03034-1#Sec1" target="_blank" >https://link.springer.com/article/10.1007/s11240-025-03034-1#Sec1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-025-03034-1" target="_blank" >10.1007/s11240-025-03034-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exploring melatonin’s influence on secondary metabolism of Echinacea purpurea callus cultures

Popis výsledku v původním jazyce

Echinacea purpurea, a key medicinal herb of the Asteraceae family, is rich in antioxidants and anti-inflammatory compounds, making it valuable in pharmaceuticals. In vitro cultures enhance sustainable metabolite production, often stimulated by elicitors. This study employed TDZ to induce E. purpurea callus cultures and assessed the impact of melatonin on biomass, antioxidant potential, and secondary metabolites. The callus cultures treated with 25 µM melatonin showed the highest biomass (FW: 208.62 g/L, DW: 11.87 g/L), total phenolic (TPP: 132.8 mg GAE/L), and flavonoid (TFP: 585.9 mg QE/L) production. Antioxidant activity peaked at this concentration, with 92.7% DPPH scavenging and 63.2 µg AAE/mg total antioxidant capacity. Moreover, the callus cultures exhibited notable enzyme inhibitory effects, with 68% inhibition against pancreatic lipase, 22% inhibition against alpha-glucosidase, and 40% inhibition against alpha-amylase, following treatment with 50 µM, 25 µM, and 10 µM of melatonin, respectively. These findings further highlight that the current approach should be further investigated to explore innovative treatments for obesity and diabetes. UPLC-MS analysis confirmed enhanced metabolite synthesis at 25–50 µM melatonin, while amino acid production peaked at 1µM. These findings highlight melatonin elicitation as an effective strategy for improving biomass, phenolics, and bioactivity in E. purpurea callus cultures.

Název v anglickém jazyce

Exploring melatonin’s influence on secondary metabolism of Echinacea purpurea callus cultures

Popis výsledku anglicky

Echinacea purpurea, a key medicinal herb of the Asteraceae family, is rich in antioxidants and anti-inflammatory compounds, making it valuable in pharmaceuticals. In vitro cultures enhance sustainable metabolite production, often stimulated by elicitors. This study employed TDZ to induce E. purpurea callus cultures and assessed the impact of melatonin on biomass, antioxidant potential, and secondary metabolites. The callus cultures treated with 25 µM melatonin showed the highest biomass (FW: 208.62 g/L, DW: 11.87 g/L), total phenolic (TPP: 132.8 mg GAE/L), and flavonoid (TFP: 585.9 mg QE/L) production. Antioxidant activity peaked at this concentration, with 92.7% DPPH scavenging and 63.2 µg AAE/mg total antioxidant capacity. Moreover, the callus cultures exhibited notable enzyme inhibitory effects, with 68% inhibition against pancreatic lipase, 22% inhibition against alpha-glucosidase, and 40% inhibition against alpha-amylase, following treatment with 50 µM, 25 µM, and 10 µM of melatonin, respectively. These findings further highlight that the current approach should be further investigated to explore innovative treatments for obesity and diabetes. UPLC-MS analysis confirmed enhanced metabolite synthesis at 25–50 µM melatonin, while amino acid production peaked at 1µM. These findings highlight melatonin elicitation as an effective strategy for improving biomass, phenolics, and bioactivity in E. purpurea callus cultures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40101 - Agriculture

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

0167-6857

Svazek periodika

161

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

—

Kód UT WoS článku

001474353000001

EID výsledku v databázi Scopus

2-s2.0-105003321126