Differentiation of Cereal Eyespot Fungi using Morphologic Characteristics and PCR Diagnostic Tests

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A_____%2F00%3A00001517" target="_blank" >RIV/60460709:_____/00:00001517 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/00:00001517

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Differentiation of Cereal Eyespot Fungi using Morphologic Characteristics and PCR Diagnostic Tests

Popis výsledku v původním jazyce

Stem parts of winter wheat infected with eyespot caused by Pseudocercosporella herpotrichoides were collected at various locations in the Czech Republic in 1995 - 1998. Pure colonies of the fungi were cultivated on potato-dextrose agar, visually, classified into the teleomorphs and tested for sensitivity to MBC-fungicides. Selected isolates of Tapesia yallundae and T. acuformis were examined using RAPD markers. Of the total 1014 isolates collected, 70% were assessed as W-type (T. yallundae) and 30% as R-type (T. acuformis). The ferquencies of T. yallundae / T. acuformis in each of the 4 years were 73/27, 78/22, 63/65 and 66/34%. That the isolates belonged to different pathotypes was proved by RAPD analysis on ten isolates that had been visually identified as the W-type and on ten of the R-type. Among five tested primers, only 5' GATGACCG 3' provided polymorphic RAPD products at minimum non-specific amplification. The genetic similarity was assessed by Dice's coefficient of similarity w

Název v anglickém jazyce

Differentiation of Cereal Eyespot Fungi using Morphologic Characteristics and PCR Diagnostic Tests

Popis výsledku anglicky

Stem parts of winter wheat infected with eyespot caused by Pseudocercosporella herpotrichoides were collected at various locations in the Czech Republic in 1995 - 1998. Pure colonies of the fungi were cultivated on potato-dextrose agar, visually, classified into the teleomorphs and tested for sensitivity to MBC-fungicides. Selected isolates of Tapesia yallundae and T. acuformis were examined using RAPD markers. Of the total 1014 isolates collected, 70% were assessed as W-type (T. yallundae) and 30% as R-type (T. acuformis). The ferquencies of T. yallundae / T. acuformis in each of the 4 years were 73/27, 78/22, 63/65 and 66/34%. That the isolates belonged to different pathotypes was proved by RAPD analysis on ten isolates that had been visually identified as the W-type and on ten of the R-type. Among five tested primers, only 5' GATGACCG 3' provided polymorphic RAPD products at minimum non-specific amplification. The genetic similarity was assessed by Dice's coefficient of similarity w

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F97%2F0636" target="_blank" >GA522/97/0636: Detekce a identifikace původce stéblolamu Pseudocercosporella herpotrichoides užitím metod polymerázové řetězové reakce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Protection Science

ISSN

1212-2580

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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