Distinguishing Isolates of Cereal Stem Disease Pathogens of the Genus Tapesia from Isolates of Fusarium and Rhizoctonia using the RAPD Method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A_____%2F00%3A00001533" target="_blank" >RIV/60460709:_____/00:00001533 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/00:00001533

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Distinguishing Isolates of Cereal Stem Disease Pathogens of the Genus Tapesia from Isolates of Fusarium and Rhizoctonia using the RAPD Method

Popis výsledku v původním jazyce

The RAPD method (Random Amplification of Polymorphous DNA) was used to distinguish Tapesia acuformis and T. yallundae isolates from other pathogens of wheat stem base diseases (Fusarium avenaceum, F. culmorum, F. nivale, Rhiozoctonia cerealis and R. solani). Isolates of the fungi originated from infected wheat plants (Triticum aestivum L.) collected at various locations in the Czech Republic. Three decameric oligonucleotide-primers were selected as producing genotype-specific RAPD products. These markers distinguish all isolates of the above taxons. The intra-species variability of these species also was studied. The values of Dice's coefficients of similarity suggest that isolates of Tapesia yallundae showed a higher degree of intra-species variability than those of T. acuformis. A high degree of intra-species variability was identified also in Fusarium avenaceum.

Název v anglickém jazyce

Distinguishing Isolates of Cereal Stem Disease Pathogens of the Genus Tapesia from Isolates of Fusarium and Rhizoctonia using the RAPD Method

Popis výsledku anglicky

The RAPD method (Random Amplification of Polymorphous DNA) was used to distinguish Tapesia acuformis and T. yallundae isolates from other pathogens of wheat stem base diseases (Fusarium avenaceum, F. culmorum, F. nivale, Rhiozoctonia cerealis and R. solani). Isolates of the fungi originated from infected wheat plants (Triticum aestivum L.) collected at various locations in the Czech Republic. Three decameric oligonucleotide-primers were selected as producing genotype-specific RAPD products. These markers distinguish all isolates of the above taxons. The intra-species variability of these species also was studied. The values of Dice's coefficients of similarity suggest that isolates of Tapesia yallundae showed a higher degree of intra-species variability than those of T. acuformis. A high degree of intra-species variability was identified also in Fusarium avenaceum.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F97%2F0636" target="_blank" >GA522/97/0636: Detekce a identifikace původce stéblolamu Pseudocercosporella herpotrichoides užitím metod polymerázové řetězové reakce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Protection Science

ISSN

1212-2580

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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