Formation and Temperature Stability of G-Quadruplex Structures Studied by Electronic and Vibration Circular Dichroism Spectroscopy Combined with Ab Initio Calculations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F08%3A00020406" target="_blank" >RIV/60461373:22310/08:00020406 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/08:00020765

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Formation and Temperature Stability of G-Quadruplex Structures Studied by Electronic and Vibration Circular Dichroism Spectroscopy Combined with Ab Initio Calculations

Popis výsledku v původním jazyce

Variations in the structure of d(GGGA)5 oligonucleotide in the presence of Li+, Na+, and K+ ions and its temperature stability were studied using electronic and vibrational circular dichroism, IR absorption, and ab initio calculations with the Becke 3-Lee-Yang-Parr functional at the 6-31G** level. The samples were characterized by nondenaturing gel electrophoresis. Oligonucleotide d(GGGA)5in the presence of Li+ forms a nonplanar single tetramer, with angles of 102° and 171° between neighboring guanine bases. This tetramer changes its geometry at temperatures >50°C, but does not form a quadruplex structure. In the presence of Na+, the d(GGGA)5 structure was optimized to almost planar tetramers with an angle of 177° between neighboring guanines. The spectral results suggest that it stacks into a quadruplex helical structure. This quadruplex structure decayed to a single tetramer at temperatures >60°C. The Hartree-Fock energies imply that d(GGGA)5 prefers to form complexes with Na+ rather

Název v anglickém jazyce

Formation and Temperature Stability of G-Quadruplex Structures Studied by Electronic and Vibration Circular Dichroism Spectroscopy Combined with Ab Initio Calculations

Popis výsledku anglicky

Variations in the structure of d(GGGA)5 oligonucleotide in the presence of Li+, Na+, and K+ ions and its temperature stability were studied using electronic and vibrational circular dichroism, IR absorption, and ab initio calculations with the Becke 3-Lee-Yang-Parr functional at the 6-31G** level. The samples were characterized by nondenaturing gel electrophoresis. Oligonucleotide d(GGGA)5in the presence of Li+ forms a nonplanar single tetramer, with angles of 102° and 171° between neighboring guanine bases. This tetramer changes its geometry at temperatures >50°C, but does not form a quadruplex structure. In the presence of Na+, the d(GGGA)5 structure was optimized to almost planar tetramers with an angle of 177° between neighboring guanines. The spectral results suggest that it stacks into a quadruplex helical structure. This quadruplex structure decayed to a single tetramer at temperatures >60°C. The Hartree-Fock energies imply that d(GGGA)5 prefers to form complexes with Na+ rather

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/OC%20135" target="_blank" >OC 135: Strukturní studie systémů inspirovaných biomolekulami pomocí pokročilých metod vibračního cirkulárního dichroismu</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biopolymers

ISSN

0006-3525

e-ISSN

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Svazek periodika

89

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000252572800006

EID výsledku v databázi Scopus

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