Tvorba a teplotní stabilita G-kvadruplexních struktur studovaných spektroskopií elektronického a vibračního cirkulárního dichroismu v kombinaci s ab initio výpočtem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F08%3A00097980" target="_blank" >RIV/68378050:_____/08:00097980 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Formation and temperature stability of G-quadruplex structures studied by electronic and vibrational dichroism spectroscopy combined with ab initio calculations

Popis výsledku v původním jazyce

Variations in the structure of d(GGGA)5 oligonucleotide in the presence of Li+, Na+, and K+ ions and its temperature stability were studied using electronic and vibrational circular dichroism, IR absorption, ab initio calculations and further characterized by nondenaturing gel electrophoresis. Oligonucleotide d(GGGA)5 in the presence of Li+ forms a nonplanar single tetramer, with angles of 102? and 171? between neighboring guanine bases. This tetramer changes its geometry at temperatures >50?C, but doesnot form a quadruplex structure. In the presence of Na+, the d(GGGA)5 structure was optimized to almost planar tetramers with an angle of 177? between neighboring guanines. The spectral results suggest that it stacks into a quadruplex helical structuredecaying to a single tetramer at temperatures >60?C. The d(GGGA)5 structure in the presence of monovalent ions is stabilized against thermal denaturation in the order Li+ < Na+ < K+.

Název v anglickém jazyce

Formation and temperature stability of G-quadruplex structures studied by electronic and vibrational dichroism spectroscopy combined with ab initio calculations

Popis výsledku anglicky

Variations in the structure of d(GGGA)5 oligonucleotide in the presence of Li+, Na+, and K+ ions and its temperature stability were studied using electronic and vibrational circular dichroism, IR absorption, ab initio calculations and further characterized by nondenaturing gel electrophoresis. Oligonucleotide d(GGGA)5 in the presence of Li+ forms a nonplanar single tetramer, with angles of 102? and 171? between neighboring guanine bases. This tetramer changes its geometry at temperatures >50?C, but doesnot form a quadruplex structure. In the presence of Na+, the d(GGGA)5 structure was optimized to almost planar tetramers with an angle of 177? between neighboring guanines. The spectral results suggest that it stacks into a quadruplex helical structuredecaying to a single tetramer at temperatures >60?C. The d(GGGA)5 structure in the presence of monovalent ions is stabilized against thermal denaturation in the order Li+ < Na+ < K+.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biopolymers

ISSN

0006-3525

e-ISSN

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Svazek periodika

89

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

144-152

Kód UT WoS článku

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EID výsledku v databázi Scopus

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