Excretion of urinary N7 guanine and N3 adenine DNA adducts in mice after inhalation of styrene

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F09%3A00022821" target="_blank" >RIV/60461373:22310/09:00022821 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/75010330:_____/09:00008406

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Excretion of urinary N7 guanine and N3 adenine DNA adducts in mice after inhalation of styrene

Popis výsledku v původním jazyce

New urinary adenine adducts, 3-(2-hydroxy-1-phenylethyl)adenine (N3alphaA), 3-(2-hydroxy-2-phenylethyl)adenine (N3betaA), were found in the urine of mice exposed to styrene vapour. These styrene 7,8-oxide derived adenine adducts as well as previously identified guanine adducts, 7-(2-hydroxy-1-phenylethyl)guanine (N7alphaG) and 7-(2-hydroxy-2-phenylethyl)guanine (N7betaG) were quantified by HPLC-ESI-MS2 and the excretion profile during and after a repeated exposure to 600 or 1200 mg/m3 of styrene for 10consecutive days (6 h/day) was determined. The excretion was dose dependent. Total N3 adenine adducts (N3alphaA + N3betaA) excreted amounted to nearly 0.8 10-5 % of the absorbed dose while urinary N7 guanine adducts (N7aplhaG + N7betaG) amounted to nearly 1.4 10-5 % of the dose. No accumulation of the adducts was observed. Due to rapid depurination from the DNA, the excretion of both N3 adenine and N7 guanine adducts ceased shortly after finishing the exposure. Both N3 adenine and N7 gua

Název v anglickém jazyce

Excretion of urinary N7 guanine and N3 adenine DNA adducts in mice after inhalation of styrene

Popis výsledku anglicky

New urinary adenine adducts, 3-(2-hydroxy-1-phenylethyl)adenine (N3alphaA), 3-(2-hydroxy-2-phenylethyl)adenine (N3betaA), were found in the urine of mice exposed to styrene vapour. These styrene 7,8-oxide derived adenine adducts as well as previously identified guanine adducts, 7-(2-hydroxy-1-phenylethyl)guanine (N7alphaG) and 7-(2-hydroxy-2-phenylethyl)guanine (N7betaG) were quantified by HPLC-ESI-MS2 and the excretion profile during and after a repeated exposure to 600 or 1200 mg/m3 of styrene for 10consecutive days (6 h/day) was determined. The excretion was dose dependent. Total N3 adenine adducts (N3alphaA + N3betaA) excreted amounted to nearly 0.8 10-5 % of the absorbed dose while urinary N7 guanine adducts (N7aplhaG + N7betaG) amounted to nearly 1.4 10-5 % of the dose. No accumulation of the adducts was observed. Due to rapid depurination from the DNA, the excretion of both N3 adenine and N7 guanine adducts ceased shortly after finishing the exposure. Both N3 adenine and N7 gua

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F06%2F0888" target="_blank" >GA203/06/0888: N7 Substituované deriváty guaninu pro časnou diagnostiku poškození DNA; vývoj syntetických a analytických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology Letters

ISSN

0378-4274

e-ISSN

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Svazek periodika

184

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000262544400006

EID výsledku v databázi Scopus

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