Urinary N3 adenine DNA adducts in humans occupationally exposed to styrene

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F10%3A00022958" target="_blank" >RIV/60461373:22310/10:00022958 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/75010330:_____/10:00009029

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Urinary N3 adenine DNA adducts in humans occupationally exposed to styrene

Popis výsledku v původním jazyce

Urine samples from humans occupationally exposed to styrene, with mandelic acid levels ranging from 400 to 1145 mg/g creatinine and from 68 to 400 mg/g creatinine for high and low exposure group, respectively, were analysed for N3 adenine DNA adducts, namely, 3-(2-hydroxy-1-phenylethyl)adenine (N3alphaA) and 3-(2-hydroxy-2-phenylethyl)adenine (N3betaA). A sensitive LC-ESI-MSMS method was developed with the limit of quantification of 1 pg/mL for both analytes. Peaks corresponding to N3alphaA and/or N3betaA were found in 7 of 9 end-of-shift samples of the high exposure group and in 6 of 19 end-of-shift samples of the low exposure group. Concentration of N3alphaA + N3betaA amounted to 2.8 +/- 1.6 pg/mL (mean +/- S.D.; n = 9) and 1.8 +/- 1.3 pg/mL (mean +/- S.D.; n = 19) in the high and low exposure group, respectively. Of other 10 samples taken the next morning after exposure, 2 contained low but quantifiable concentrations of N3alphaA and none contained N3betaA. However, interfering peak

Název v anglickém jazyce

Urinary N3 adenine DNA adducts in humans occupationally exposed to styrene

Popis výsledku anglicky

Urine samples from humans occupationally exposed to styrene, with mandelic acid levels ranging from 400 to 1145 mg/g creatinine and from 68 to 400 mg/g creatinine for high and low exposure group, respectively, were analysed for N3 adenine DNA adducts, namely, 3-(2-hydroxy-1-phenylethyl)adenine (N3alphaA) and 3-(2-hydroxy-2-phenylethyl)adenine (N3betaA). A sensitive LC-ESI-MSMS method was developed with the limit of quantification of 1 pg/mL for both analytes. Peaks corresponding to N3alphaA and/or N3betaA were found in 7 of 9 end-of-shift samples of the high exposure group and in 6 of 19 end-of-shift samples of the low exposure group. Concentration of N3alphaA + N3betaA amounted to 2.8 +/- 1.6 pg/mL (mean +/- S.D.; n = 9) and 1.8 +/- 1.3 pg/mL (mean +/- S.D.; n = 19) in the high and low exposure group, respectively. Of other 10 samples taken the next morning after exposure, 2 contained low but quantifiable concentrations of N3alphaA and none contained N3betaA. However, interfering peak

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/2B08051" target="_blank" >2B08051: Nová strategie biologického monitorování exposice mutagenům a karcinogenům</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology Letters

ISSN

0378-4274

e-ISSN

—

Svazek periodika

197

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

5

Strana od-do

—

Kód UT WoS článku

000281002700005

EID výsledku v databázi Scopus

—